We narrowed to 9,389 results for: Coli
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Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
RSV-betaGal
Plasmid#24058DepositorInsertLacZ (lacZ E. coli)
UseTagsRSV promoterExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM901
Plasmid#72966PurposeLevel 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM901 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS2
Plasmid#191123PurposeTranscription of 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertMS2
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMYO127TAG-SepT
Plasmid#34625DepositorInsertMyoglobin-127TAG
UseTags6xHISExpressionBacterialMutationcodon-optimized + an amber stop codon was introdu…PromoterIppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLacZ-LucWm1A
Plasmid#44167DepositorInsertsLacZ
firefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB, CMV enhancer/chicken beta-actin promoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)
Plasmid#70198PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEINO5
Plasmid#179489PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertaacC1
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCO706
Plasmid#139743PurposeIndividual component of Caffeic Acid biosynthesis pathway, hpaCDepositorInserthpaB
UseMoclo level 0 componentTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCO707
Plasmid#139744PurposeIndividual component of Caffeic Acid biosynthesis pathway, hpaCDepositorInserthpaC
UseMoclo level 0 componentTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDrop
Plasmid#184872PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAL3
Plasmid#114673PurposeT7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.DepositorTypeEmpty backboneUseTagsoptional Ser-Gly-6HisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
DVK_AF
Plasmid#66068PurposeMoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG/Q157TAG
Plasmid#68299PurposeE17/Q157 TAG GFP reporterDepositorInsertGFP E17TAG/Q157TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLBC0151
Plasmid#172796PurposeLevel 0 module containing CT_TurboIDDepositorInsertTurboID with 3XHA tag
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
UseTagsExpressionBacterialMutationPromoterlacIqAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinD-IRES-Puro
Plasmid#213114PurposeExpression of untagged MinD in mammalian cellsDepositorInsertMinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT005
Plasmid#177323PurposeMCP-SoxS (R93A) expressionDepositorInsertSoxS
UseTagsMCP (MS2 Coat Protein)ExpressionMutationR93APromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
UseTags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D-5
Plasmid#107865PurposeBacterial expression of PIS and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p005kanRFP
Plasmid#58533PurposeRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-600u
Plasmid#13563DepositorInsertFLIPglu F16A Y12S-ECFP-D13R
UseTagsCitrineExpressionBacterialMutationF16A ECFP insertion in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a-RFP
Plasmid#72958PurposeLevel 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-ABDepositorInsertLevel 2 - a site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-IC15
Plasmid#165408PurposeBacteria gene circuit on medium copy backbone.DepositorInsertIC15 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISP_IS
Plasmid#120425PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterconstitutiveAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
tet operator
Plasmid#8901DepositorInserttet operator
UseTagsExpressionMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-2
Plasmid#59945Purposedihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorInsertsdihydropterine reductase
pterin-4a-carbinolamine dehydratase
UseTagsExpressionMutationPromoterpTrcAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
E17TAG GFP zeo resistance
Plasmid#69118PurposeFor E17TAG GFP reporter expression in non-zeocin resistant strainsDepositorInsertE17TAG GFP
UseTagsHisExpressionBacterialMutationE17 to TAGPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-3.2mDelta13
Plasmid#13560DepositorInsertFLIPglu F16A S112A Delta13
UseTagsECFP and EYFPExpressionBacterialMutationF16A, S112A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-RFP
Plasmid#72940PurposeLevel 1 Destination vector - Position BDepositorInsertLevel 1 - B site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXM03
Plasmid#121150PurposeThis plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coliDepositorInsertXimB, IDS2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
SepOTSκ
Plasmid#68291PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 4xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092686
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET FLII275Pglu-4.6m
Plasmid#13565DepositorInsertFLIPglu F16A G275S-ECFP-K276R
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only