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167,150 results

Showing: 3401 - 3440 of 167150 results

  2. pJL-TRBO-G

    Plasmid
    #80083
    Purpose
    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Insert
    GFP
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Nov. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    80083-plasmid-map-sequence-id-372997

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  3. pAAV.cTNT.iCre

    Plasmid
    #69916
    Purpose
    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.
    Depositor
    William Pu
    Article
    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:
    Has Service
    AAV9
    Insert
    improved cre (iCre) recombinase and tdTomato
    Use
    AAV
    Tags
    The iCre coding frame and tdTomato coding frame i…
    Promoter
    Chicken cardiac troponin T
    Available Since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    69916-plasmid-map-sequence-id-472137

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  5. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

    Plasmid
    #128450
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128450-plasmid-map-sequence-id-256464

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  8. pcDNA3.1-C-Luc

    Plasmid
    #185358
    Purpose
    For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    185358-plasmid-map-sequence-id-376433

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  9. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  12. FURIN-bio-His

    Plasmid
    #51755
    Purpose
    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FURIN (FURIN Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    51755-plasmid-map-sequence-id-79339

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  13. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Promoter
    AOX1
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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  16. Lenti-pHSE::Cre-mRuby3

    Plasmid
    #164136
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Insert
    HSE promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Expression
    Mammalian
    Promoter
    HSE synthetic promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164136-plasmid-map-sequence-id-323779

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  19. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  21. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  23. pAG CMV Empty Puro

    Plasmid
    #237881
    Purpose
    Empty mammalian expression plasmid for cloning untagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237881-plasmid-map-sequence-id-469912

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  24. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

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  25. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid
    #220349
    Purpose
    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
    Depositor
    Tomoko Igawa
    Article
    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.
    Inserts
    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    Tags
    Superman Repression Domain X (SRDX) and VP16 tran…
    Expression
    Plant
    Mutation
    two amino acid substitution in WUS-box: L255A L25…
    Promoter
    CaMV 35S and RPS5A
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220349-plasmid-map-sequence-id-440868

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  27. mCit-APPAPAPPAP-gs

    Plasmid
    #228091
    Purpose
    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, APPAPAPPAP
    Expression
    Mammalian
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228091-plasmid-map-sequence-id-451163

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  28. PSMA4_GFP_Cterm

    Plasmid
    #221514
    Purpose
    Fluorescently tagged Psma4-GFP
    Depositor
    Alessandro Ori
    Insert
    PSMA4 (PSMA4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221514-plasmid-map-sequence-id-439377

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Showing: 3401 - 3440 of 167150 results