19,623 results

1. pCMV-Luc

   Plasmid

   #45968

   Depositor

   Ralf Kuehn

   Insert

   Luciferase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-Tet-On-shRNA-Control

   Plasmid

   #98398

   Purpose

   Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC-tag-N

   Plasmid

   #108078

   Purpose

   Gateway compatible destination vectors containing StrepIII/HA/BioID

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CCDB (ccdB(letD) )
   

   Tags

   StrepIII-HA-BirA*

   Expression

   Bacterial and Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV.CBA.eGFP.2A.P301L-Tau

   Plasmid

   #140425

   Purpose

   AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

   Depositor

   Bradley Hyman

   Article

   Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

   Insert

   eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
   

   Use

   AAV

   Tags

   eGFP

   Mutation

   changed Proline 301 to Leucine

   Promoter

   CBA

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Ub

   Plasmid

   #11928

   Purpose

   Used for in vivo monitoring of ubiquitin localization.

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin (UBC Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-3XHA-EGFP-OMP25

   Plasmid

   #83356

   Purpose

   For tagging mitochondria with HA epitopes (HA-MITO construct)

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XHA-EGFP-OMP25
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti Lifeact-iRFP670 BlastR

   Plasmid

   #84385

   Purpose

   Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   iRFP670

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ub-R-GFP

   Plasmid

   #11939

   Depositor

   Nico Dantuma

   Article

   Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

   Insert

   Ub-R-GFP
   

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Arginine at…

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PEmax-P2A-hMLH1dn

   Plasmid

   #174828

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p53-GFP

    Plasmid

    #11770

    Depositor

    Geoff Wahl

    Insert

    p53 (TP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    72R variant of p53

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. mCherry-TOMM20-N-10

    Plasmid

    #55146

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-blast

    Plasmid

    #26655

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLysscys255

    Plasmid

    #36429

    Depositor

    Pete Lollar, Emine Gunhan

    Article

    Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

    Insert

    Saporin-3/cys255
    

    Expression

    Bacterial

    Mutation

    cys added at position 255

    Promoter

    T7

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-Nme2ABE8e

    Plasmid

    #189928

    Purpose

    Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    Nme2ABE8e
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HPSE-bio-His

    Plasmid

    #53407

    Purpose

    Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    HPSE (HPSE Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-eGFP

    Plasmid

    #36083

    Purpose

    3rd generation lentiviral transfer plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ef1a_Large T-antigen_Ires_Puro

    Plasmid

    #18922

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    Large T Antigen (SV40gp6 Simian virus 40)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-FLAG-SF3B1-WT

    Plasmid

    #82576

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 (wild-type)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-WT (SF3B1 Human)
    

    Tags

    FLAG peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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