159,284 results

1. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLNHA-C1-Hs4E-BP1

   Plasmid

   #79438

   Purpose

   Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Hs4E-BP1 (EIF4EBP1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEP116E

   Plasmid

   #97402

   Purpose

   binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Plasma membrane target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. EFR WT

   Plasmid

   #102413

   Purpose

   fluorescent tagging. Plant expression of EFR WT

   Depositor

   Silke Robatzek

   Insert

   EFR (EFR Mustard Weed)
   

   Use

   Tags

   6xHis and mgfp5

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_NEUROG3_P2A_Hygro_Barcode

   Plasmid

   #120468

   Purpose

   Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NEUROG3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXRM15

   Plasmid

   #63150

   Purpose

   Met15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

   Depositor

   Marc Gartenberg

   Article

   Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p35S::AtSOBIR1-mCherry

   Plasmid

   #86166

   Purpose

   SOBIR1-mCherry expression

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS/D614G

   Plasmid

   #164566

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS-D614G variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCXIZ GFP C1

   Plasmid

   #202424

   Purpose

   Encodes GFP alone

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIZ GFP PODXL delta EC

    Plasmid

    #202423

    Purpose

    Expression of GFP-tagged PODXL without extracellular domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    deleted extracellular domain

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCXIZ GFP PODXL 5N>Q

    Plasmid

    #202419

    Purpose

    Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    N33/43/104/144/360Q

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB2796

    Plasmid

    #63641

    Purpose

    pCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTagRFP-T-Paxillin-22

    Plasmid

    #58024

    Purpose

    Localization: Focal Adhesions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Paxillin
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR223-ULK4

    Plasmid

    #23849

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ULK4 (ULK4 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pLO99

    Plasmid

    #90219

    Purpose

    Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_03691
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR8/GW/TOPO-TBX20a

    Plasmid

    #98620

    Purpose

    Gateway entry TOPO TA cloning vector for TBX20a

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    Tbx20a (Tbx20 Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLNHA-C1-Hs4E-BP3

    Plasmid

    #79439

    Purpose

    Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4E-BP3 (EIF4EBP3 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-Hygro-TRE>{MCS}:T2A:EGFP

    Plasmid

    #236250

    Purpose

    Hygromycin selected lentiviral vector with MCS for tetracycline-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA HA DUSP6

    Plasmid

    #236430

    Purpose

    transient overexpression of DUSP6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-PM-ExRai-CKAR2

    Plasmid

    #236098

    Purpose

    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    Lyn-ExRai-CKAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. plenti-ITGB6-HA

    Plasmid

    #235242

    Purpose

    Lentiviral vector to generate HA-tagged human ITGB6 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

    Insert

    ITGB6 (ITGB6 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAG-FLEX-MCS-WPRE-SV40 - GCH2

    Plasmid

    #230815

    Purpose

    AAV cloning vector for CRE dependent expression under the CAG promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #232929

    Purpose

    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    OsTir1(F74G)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 74 to Glycine

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGEN-PspCas13b-NES-mCherry

    Plasmid

    #233608

    Purpose

    Expression of PspCas13b in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    PspCas13b-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC016-gPsp-EGFP_start_codon

    Plasmid

    #233613

    Purpose

    Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    gRNA targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

    Plasmid

    #221329

    Purpose

    Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    TRE3G

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-Mch-MAP1LC3B

    Plasmid

    #201924

    Purpose

    For lentiviral transduction of Mch-MAP1LC3B

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Pan-QKI scFv [N147/6]

    Plasmid

    #211605

    Purpose

    Mammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG/ATP10D(E215Q)-HA

    Plasmid

    #209248

    Purpose

    Mammalian expression of ATP10D mutant E215Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E215Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge