We narrowed to 13,730 results for: TIM

1. pTrichoGate-13

   Plasmid

   #160713

   Purpose

   For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

   Plasmid

   #138989

   Purpose

   IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCNL-N1_Mito

   Plasmid

   #65670

   Purpose

   Expresses Mito-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   cytochrome c oxidase subunit VIII
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pN1-mCherry-NKD2

   Plasmid

   #176641

   Purpose

   Expresses NKD2-mCherry fusion protein in mammalian cells

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   mCherry tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ERrefGFP1_pCDNA3

   Plasmid

   #48635

   Purpose

   Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERrefGFP1
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C147S,C204Q

   Promoter

   Available since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

   Plasmid

   #169370

   Purpose

   Expression of gRNA in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CPIJ039596

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ptagBFP-TetR-NLS-VP16

   Plasmid

   #103835

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-TetR-NLS-VP16 (tetR )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1X-Nup88-mut

   Plasmid

   #87337

   Purpose

   To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-ChroME(F243Y)-mRuby2-ST

   Plasmid

   #109127

   Purpose

   Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChroME(F243Y)-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCNL-C1_RhoB

    Plasmid

    #65697

    Purpose

    Expresses CNL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. XE97 mFZ8-CS2+

    Plasmid

    #16800

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    mFZ8-CS2+ (Fzd8 Mouse)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pONL-N1_Golgi

    Plasmid

    #65695

    Purpose

    Expresses Golgi-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    N-terminal 81 amino acids of beta 1,4-galactosyltransferase
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCNL-N1_ITPKA

    Plasmid

    #65688

    Purpose

    Expresses ITPKA-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    inositol triphosphate 3-kinase A
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    contains residues 9-52

    Promoter

    CMV

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYNL-N1_zyxin

    Plasmid

    #65681

    Purpose

    Expresses Zyxin-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    zyxin (Zyx Mouse)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

15. roGFP_iE_C204S_pQE30

    Plasmid

    #48632

    Purpose

    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    roGFP1
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    insertion E147B, c204S

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCNL-N1_3xNLS

    Plasmid

    #65673

    Purpose

    Expresses NLS-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOGG113

    Plasmid

    #133124

    Purpose

    pL0M-PU-NoP, promotorless region used as negative control

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    NoP promotorless region from plasmid pIJJ11268
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYNL-N1_3xNLS

    Plasmid

    #65672

    Purpose

    Expresses NLS-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-kanMX6-Purg1-GST

    Plasmid

    #39352

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GST and kanMX6 module - reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pNB695

    Plasmid

    #60144

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNB697

    Plasmid

    #60146

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS/U6/hTID1

    Plasmid

    #13745

    Depositor

    Karl Munger

    Article

    Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

    Insert

    hTid1 siRNA (DNAJA3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Inverted repeat of 21 bp with 6 nuc. spacer.

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

23. T7T3-PacI-Neto1

    Plasmid

    #87672

    Purpose

    In situ probe for mouse Neto1

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Neto1 (Neto1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial sequence, (1244 to 2278bp of NM_144946.4)

    Promoter

    T7/T3

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYNL-N1_Mito

    Plasmid

    #65669

    Purpose

    Expresses Mito-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    cytochrome c oxidase subunit VIII
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pONL-N1_zyxin

    Plasmid

    #65683

    Purpose

    Expresses Zyxin-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    zyxin (Zyx Mouse)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pONL-N1_3xNLS

    Plasmid

    #65674

    Purpose

    Expresses NLS-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pONL-N1_TUBB5

    Plasmid

    #65680

    Purpose

    Expresses beta-tubulin-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    beta-tubulin class V
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pCas12a-NT

    Plasmid

    #235167

    Purpose

    Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA

    Depositor

    Constantinos Patinios

    Article

    Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

    Insert

    FnCas12a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSIBR2.0-818-Cas12a-NT

    Plasmid

    #235168

    Purpose

    Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

    Depositor

    Constantinos Patinios

    Article

    Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

    Insert

    SIBR2.0-818-Cas12a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SP_C3

    Plasmid

    #228159

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_Q03178_PIR1_YEAST:0-18
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIDTsmart-RC2-delVP2-VP2-454TAG

    Plasmid

    #240060

    Purpose

    Express AAV2 capsid with ncAA mutant at site 454 of VP2

    Depositor

    Abhishek Chatterjee

    Article

    Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

    Inserts

    RC2-DelVP2
    VP2-454-TAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIDTsmart-RC2-delVP1-VP1-454TAG

    Plasmid

    #240059

    Purpose

    Express AAV2 capsid with ncAA mutant at site 454 of VP1

    Depositor

    Abhishek Chatterjee

    Article

    Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

    Inserts

    RC2-DelVP1
    VP1-454-TAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ptet-lux-Tn7T-Gm

    Plasmid

    #226434

    Purpose

    This vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.

    Depositor

    Xue Liu

    Article

    Zhang et al bioRxiv 2024.09.01.610675

    Inserts

    optimized TetR
    luxABCDE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcon and Ptet

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIND-GLucZ

    Plasmid

    #228704

    Purpose

    Inducible expression of Gaussia luciferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pDIV299

    Plasmid

    #204967

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC-hU6-2O-crScaffold_SV40-BFP

    Plasmid

    #224861

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC-hU6-2O-crScaffold_EF1a-BFP

    Plasmid

    #224862

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LPUtopia-8

    Plasmid

    #224781

    Purpose

    Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Inserts

    puromycin N-acetyltransferase
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a- mCherry-FIVAR-HIS-ybbR

    Plasmid

    #169138

    Purpose

    E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    mCherry
    

    Use

    Tags

    6x Histag, FIVAR tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMRS-BG42-GFP

    Plasmid

    #220202

    Purpose

    mini-Tn7 GFP expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge