19,738 results

1. pRiA4-VIR

   Plasmid

   #138193

   Purpose

   Virulence helper compatible with pCambia binary vectors.

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   vir gene cluster
   

   Use

   Vir gene expression

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pENN.AAV.hSyn.Cre.WPRE.hGH

   Plasmid

   #105553

   Purpose

   AAV expression of Cre from hSyn promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSGV1-1D3-28Z.1-3 mut

   Plasmid

   #107227

   Purpose

   Anti-mouse CD19 CAR with CD3 ITAMs mutated

   Depositor

   James Kochenderfer, Steven Rosenberg

   Article

   Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

   Insert

   1D3-28Z. 1-3 mut
   

   Use

   Retroviral

   Tags

   None

   Mutation

   None

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lentiviral-TOP-dGFP-reporter

   Plasmid

   #14715

   Purpose

   Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   LEF-1/TCF-responsive promoter (Ctnnb1 )
   

   Use

   Lentiviral

   Tags

   d2 eGFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI HA NEDD4

   Plasmid

   #27002

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   NEDD4 (NEDD4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-dTAG-Puro

   Plasmid

   #207727

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. TUBB5-Halo

   Plasmid

   #64691

   Purpose

   Expresses beta-tubulin labeled with Halo-tag

   Depositor

   Yasushi Okada

   Article

   Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

   Insert

   TUBB
   

   Tags

   Halo-tag

   Expression

   Mammalian

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRFP-Rab5

    Plasmid

    #14437

    Purpose

    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 5 (RAB5A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDAC565

    Plasmid

    #195242

    Purpose

    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry2-N1

    Plasmid

    #54517

    Purpose

    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPRx-Sth1Cas9-L5

    Plasmid

    #140993

    Purpose

    Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

    Depositor

    Wilbert Bitter

    Article

    Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

    Inserts

    Sth1Cas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified; Integrative in mycobactreia

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

    Plasmid

    #153004

    Purpose

    Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (N) (G78)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVHA E2F1

    Plasmid

    #24225

    Purpose

    Mammalian expression of human E2F1 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F1 (E2F1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDest-527

    Plasmid

    #11518

    Purpose

    Gateway destination vector for bacterial expression; His tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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