We narrowed to 8,544 results for: sgrna

1. py100-sgRNA_hVEGFA

   Plasmid

   #198282

   Purpose

   Expression of SpCas9 guide RNA specific for human VEGFA locus

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   py100-sgRNA_hVEGFA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT7-th-sgRNA

   Plasmid

   #65565

   Purpose

   in vitro trancription of sgRNA targeting the zebrafish th locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   th sgRNA (th Zebrafish)
   

   Use

   In vitro rna transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-U6-AasgRNA

   Plasmid

   #121954

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b single chimeric gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LENTICRISPR-TPI1_sgRNA1

   Plasmid

   #201626

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   TPI1 (TPI1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LENTICRISPR-TPI1_sgRNA2

   Plasmid

   #201627

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   TPI1 (TPI1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-U6-AasgRNA3.8

   Plasmid

   #121958

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b single chimeric gRNA, short version
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLH-stsgRNA2.1

   Plasmid

   #64117

   Purpose

   Vector for expression of St1 sgRNA2.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cas12c-sgRNA_BbsI_CAM

   Plasmid

   #183221

   Purpose

   Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psgRNA-ccdB

   Plasmid

   #99915

   Purpose

   Basic cloning vector for the expression of imaging-based optimization sgRNA

   Depositor

   Yujie Sun

   Article

   Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOsU3-esgRNA

    Plasmid

    #115629

    Purpose

    expression esgRNA in rice protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    OsU3p-esgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Neg. Ctrl_sgRNA

    Plasmid

    #111298

    Purpose

    CRISPR KO murine neg. ctrl.

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    NegCtrl sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

    Plasmid

    #214814

    Purpose

    A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

    Depositor

    Yannick Doyon

    Insert

    St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LENTICRISPR-PGAM1_sgRNA2

    Plasmid

    #201615

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LENTICRISPR-HNRNPD_sgRNA1

    Plasmid

    #201598

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HNRNPD (HNRNPD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PAX6 sgRNA7

    Plasmid

    #68466

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX458-sgRNA_Ago1_1

    Plasmid

    #73533

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX458-sgRNA_Ago1_2

    Plasmid

    #73534

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

19. LENTICRISPR-GPI_sgRNA1

    Plasmid

    #201594

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97309

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LENTICRISPR-ALDOA_sgRNA2

    Plasmid

    #201591

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    ALDOA (ALDOA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLH-stsgRNA1.1

    Plasmid

    #64116

    Purpose

    Vector for expression of St1 sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LENTICRISPR-HK2_sgRNA2

    Plasmid

    #201597

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX458-sgRNA_Ago2_3

    Plasmid

    #73531

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRRAP sgRNA1

    Plasmid

    #138182

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRRAP sgRNA3

    Plasmid

    #138184

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TRRAP sgRNA2

    Plasmid

    #138183

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRRAP

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TRRAP (TRRAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX458-sgRNA_Ago2_4

    Plasmid

    #73532

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LENTICRISPR-PABPC1_sgRNA1

    Plasmid

    #201608

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. lentiCRISPR v2-Nr4a1sgRNA1

    Plasmid

    #160945

    Purpose

    Guide RNA 1 to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 gRNA 1 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. double_sgRNA targeting e3 and e7

    Plasmid

    #190689

    Purpose

    sgRNAs targeting enhancer 3 and 7 of MYC separately

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNAs targeting enhancer 3 and 7 of MYC separately
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PAX6 sgRNA2

    Plasmid

    #68465

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKDsgRNA-ldhA

    Plasmid

    #102288

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ldhA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid

    #178107

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick exon-53 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Construct 1 - U6p_20nt1_WT_sgRNA

    Plasmid

    #81245

    Purpose

    For assaying self-targeting activity via Cas9

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    sgRNA
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBbdCas9S_P0526-sgRNA500

    Plasmid

    #149641

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBSV2G_PflaBS-sgRNA500

    Plasmid

    #149615

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaBS

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge