We narrowed to 167,150 results for: Gene

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Para

Plasmid

#218363

Purpose

Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOT563

Plasmid

#40864

Depositor

Harold Gainer

Article

Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

Insert

Oxytocin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pT1QciMP

Plasmid

#62216

Purpose

QUAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Promoter

QUAS-E1b

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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p159 pPr-luc

Plasmid

#8392

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin promoter (Pbsn Rat)

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only
pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pXWS320tetO

Plasmid

#160830

Purpose

320 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(320 X tetO)-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR U6-hTERTsh

Plasmid

#111385

Purpose

Knock down of hTERT transcript

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

shRNA C3 against hTERT

Use

Gateway entry cloning

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBFv-U6.2B

Plasmid

#138401

Purpose

gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

U6-2

Expression

Insect

Promoter

U6-2

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMTB

Plasmid

#112225

Purpose

Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

Depositor

Sean Megason

Article

Wagner et al Science. 2018 Apr 26. pii: science.aar43

Insert

Super-folder GFP

Use

Zebrafish expression

Promoter

beta actin 2 (zebrafish)

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.1Uw

Plasmid

#26226

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

Plasmid

#183027

Purpose

B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/Onc

Plasmid

#21360

Depositor

David Largaespada

Article

Collier et al Nature. 2005 Jul 14. 436(7048):272-6.

Insert

Sleeping Beauty Transposon with splice acceptors and murine stem cell virus LTR

Use

Transposon

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pPEPZ-Plac

Plasmid

#122635

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM112

Plasmid

#127229

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2820

Plasmid

#160669

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_TKT-Halo

Plasmid

#175331

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

TKT (TKT Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-588TAG

Plasmid

#240122

Purpose

Express AAV2 capsid with ncAA mutant at site 588 of VP1

Depositor

Abhishek Chatterjee

Article

Pham et al bioRxiv 2024.07.20.604406

Inserts

RC2-DelVP1
VP1-588-TAG

Use

AAV

Expression

Mammalian

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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GA_HBV_MS2_(AmpR)

Plasmid

#179155

Purpose

Encodes the X, C and S genes of Hepatitis B Virus

Depositor

Paul Freemont

Article

Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

Insert

XCS Gene

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pB6-Gfap-T2A-tdTomato-FNF-DTA

Plasmid

#140271

Purpose

B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Gfap (Gfap Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tdTomato

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFlpStop-HDR-UAS-2.1-tdTom

Plasmid

#89148

Purpose

FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

Depositor

Tom Clandinin

Article

Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

5XUAS, 3XP3-dsRed

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKESK22

Plasmid

#64857

Purpose

expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

Depositor

Karin Schnetz

Article

Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

Type

Empty backbone

Expression

Bacterial

Promoter

Ptac

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-Cre

Plasmid

#183425

Purpose

Lentiviral Cre vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-Cre

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[AV-60]

Plasmid

#67012

Purpose

Expresses malE-ELP[AV-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[AV-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBI-p73 wt/EGFP

Plasmid

#16545

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p73 (TP73 Human)

Tags

HA

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pDD132

Plasmid

#119885

Purpose

Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

BbaJ23104_EYFP

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVA.008

Plasmid

#31809

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

JRTCLCrVA.008

Use

Agrobacterium binary

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pKM402

Plasmid

#107770

Purpose

Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Inserts

Che9C RecT
TetR (tet repressor)
cat
kan

Expression

Bacterial

Promoter

Cat promoter, Ptet, kan promoter, and pTB21

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1A3-AD011

Plasmid

#116852

Purpose

Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Ph

Plasmid

#46814

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-H-mphR

Plasmid

#62559

Purpose

Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-H-araC

Plasmid

#62563

Purpose

Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p155 pCMV-EGFP/beta-gal

Plasmid

#8387

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAS9-BFP empty

Plasmid

#80976

Purpose

Cas9, TagBFP, and sgRNA expression

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDC.154

Plasmid

#222228

Purpose

Acceptor plasmid for human retron-Eco1 variants.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Type

Empty backbone

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEV-G2-Km

Plasmid

#46815

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c5X-ELP[V-60]

Plasmid

#67013

Purpose

Expresses malE-ELP[V-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSXM2-pLacI-GFP

Plasmid

#174617

Purpose

Bacterial expression of GFP.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLacI

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC8-40XUAS-IVS-mCD8::GFP

Plasmid

#26221

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Promoter

none

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CS2 HA-Smad6

Plasmid

#14962

Depositor

Joan Massague

Article

Hata et al Genes Dev. 1998 Jan 15. 12(2):186-97.

Insert

Smad6 (SMAD6 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

HA

Mutation

S18L

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-mito

Plasmid

#71542

Purpose

Biomedical Research

Depositor

David Sidransky

Article

Dasgupta et al Cancer Res. 2008 Feb 1;68(3):700-6. doi:

Insert

myc-mito

Use

Cytomegalovirus

Tags

none

Promoter

CMV

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pJH674_pTech_PlldR-HHRz-supP

Plasmid

#163728

Purpose

Lactate-inducible expression of supP, preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GG-dest

Plasmid

#69538

Purpose

Destination plasmid for gRNA concatenation Golden Gate cloning

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Type

Empty backbone

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB50

Plasmid

#101678

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 167,150 results for: Gene