We narrowed to 8,039 results for: Dos;
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Plasmid#192462PurposeExpression of RvCAHS12-GS_linker-AcGFP1 in mammalian cellsDepositorInsertCAHS12
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
Plasmid#78101PurposeExpresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-EED (EED Synthetic, Human)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-hCdt1(1-100)ΔCy
Plasmid#193759PurposeExpresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)DepositorInsertmCherry-hCdt1(1-100)ΔCy (CDT1 Human)
UseLentiviralTagsExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterEF1aAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eArch 3.0-EYFP
Plasmid#35516PurposeAAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.DepositorInsertArch3.0-EYFP
UseAAVTagsEYFPExpressionMammalianMutationEnhanced trafficking signal and ER export signal …PromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY30
Plasmid#84745PurposeExpresses huAsCpf1-P2A-puro and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q23-Myc-B
Plasmid#110488PurposeLentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationshort 3'UTR mutant huntingtin exon1 fragment…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SLC38A9.1
Plasmid#71869PurposeoverexpressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseTagsHAExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
IL2 pMalLP
Plasmid#41808DepositorInsertIL2 (IL2 Human)
UseTags3C protease site, 6x His, and Maltose Binding Pro…ExpressionBacterialMutationPromoterpTacAvailable sinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI3_G-alpha
Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA1-FLAG
Plasmid#79678Purposeserotonin receptor 2C RNA isoform 1 with C-terminal flag tagDepositorInsert5HT2C-RNA1 (HTR2C Human)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82705PurposeAAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertU6/TO gRNA Expression Cassette Containing Tet2 gRNA
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
His10-CD3z-SNAPf-ybbR (XSB507)
Plasmid#78508PurposeConstruct for expressing CD3z-SNAPf-ybbR in E. ColiDepositorInsertCD3z (Cytoplasmic domain) (CD247 Human)
UseTagsHis10 and SNAPf-ybbRExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_USP7_MATH
Plasmid#178490PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertUSP7_MATH (USP7 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4203
Plasmid#200160PurposePrig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE
Plasmid#122056PurposeLentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253PDepositorUseLentiviralTagsExpressionMammalianMutationMyoD chimeric protein substituted with the NeuroD…PromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82706PurposeAAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertU6/TO Empty gRNA Expression Cassette
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-C-CreintG
Plasmid#69571PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - C-CreintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP
Plasmid#155306PurposeRfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFPDepositorInsertRfx Cas13d CRISPR RNA BFP
UseTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IKZF1-V2-V5
Plasmid#107390PurposeMammalian expression of IKZF1-V2-V5DepositorInsertIKZF1-V2 (IKZF1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82704PurposeAAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertH1/TO-L Empty gRNA Expression Cassette
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRT_htr2c(c)-GFP
Plasmid#79677Purposeserotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFPDepositorInsertserotonin receptor 2C (HTR2C Human)
UseTagsEGFP in FrameExpressionMammalianMutationsplice site of exon Vb in consensusPromoterCMVAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-human LRRC33
Plasmid#111602PurposeExpress human LRRC33 in 293T cellsDepositorInserthuman LRRC33 (NRROS Human)
UseTagsmyc/his tagExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY026
Plasmid#84741PurposeExpresses huAsCpf1 and crRNA guideDepositorInserthuAsCpf1
UseTags3xHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-CyOFP1
Plasmid#105799PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-CyOFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
Plasmid#35512PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q145-Myc-B
Plasmid#110489PurposeLentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationpartial long 3'UTR wild type huntingtin exon…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe JUND-HA neo
Plasmid#58489Purposemammalian expression of human JUNDDepositorInsertJUND (JUN Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-empty-DSE-TetO-8kb
Plasmid#213022PurposepiggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposaseDepositorInsertTetO
UseMouse Targeting; Piggybase integration (transposo…TagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC6
Plasmid#224346PurposeExpresses human KDAC6 (HDAC6) in insect cellsDepositorInsertKDAC6 (HDAC6 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lentiviral-His-tagged-PSPH
Plasmid#134786PurposeStable overexpression lentiviral vector of His tagged PSPHDepositorInsertPhosphoserine phosphatase (PSPH Human)
UseLentiviralTagsHisExpressionMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
psd44-iGFP-MFGE8-long
Plasmid#46847Purposeexpression of MFGE8 in mammalian cellsDepositorInsertMFGE8 (Mfge8 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1P-iCLHN
Plasmid#66579PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENsDepositorInsertNanoluc-HaloTag
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-DDX54-FLAG/HA
Plasmid#97062PurposeExpresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti-px330-CRBN-T1-pGK-Pur
Plasmid#107382PurposeMammalian expression CRISPR/Cas9DepositorInsertsgRNA targeting CRBN (CRBN Human)
UseLentiviral; CrisprTagsExpressionMammalianMutationPromoterU6Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EphB3-Fc
Plasmid#200985PurposeMammalian expression plasmid for soluble EphB3 fused to IgG1 FcDepositorInsertEphB3 (EPHB3 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationSoluble ectodomain of EphB3PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH1R-P2A-mCherry-N1
Plasmid#84330PurposeCo-expresses (untagged) Histamine-1 receptor and mCherryDepositorInsertH1R-P2A-mCherry (HRH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CACNB1_Guanylate-kin
Plasmid#110036PurposeProtein expression and purification of CACNB1_Guanylate-kinDepositorInsertCACNB1_Guanylate-kin (CACNB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
Plasmid#35511PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY31
Plasmid#84746PurposeExpresses huLbCpf1-P2A-puro and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseCRISPRTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
SOX17-3'gRNA
Plasmid#210465PurposegRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertSOX17 (SOX17 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1ΔCy-mCherry-Puro
Plasmid#193764PurposeDoxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1ΔCy-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationCy motif (a.a. 68-70) mutated to alanine, a.a. 11…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR
Plasmid#160046PurposeFor expressing the full paradoxical circuitDepositorInsertsosTIR1
iaaH
iCasp9
BlastR
UseSynthetic BiologyTagsmAID, mCherry, and mGFPmut3ExpressionMammalianMutationPromoterno promoter, T2A is used and pEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC13P-iCLHN
Plasmid#66580PurposeCLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENsDepositorInsertNanoluc-HaloTag
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only