Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 173 results for: pef gfp

Clear keywords
Showing: 1 - 50 of 173 results

  4. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid
    #71719
    Purpose
    Expressing a GFP-tagged degron in the soma of C. elegans
    Depositor
    Abby Dernburg
    Publication
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    Tags
    EmGFP
    Expression
    Worm
    Mutation
    minimal degron sequence (71-114aa) with start cod…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71719-plasmid-map-sequence-id-246098

    Enlarge

  11. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138272-plasmid-map-sequence-id-273617

    Enlarge

  13. pEF5B-FRT-control-APEX

    Plasmid
    #73187
    Purpose
    Expresses "control"-APEX (cytosolic) in mammalian cells
    Depositor
    Maxence Nachury
    Publication
    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    control-APEX (Nphp3 Mouse, Glycine max)
    Tags
    internal GFP tag
    Expression
    Mammalian
    Mutation
    changed glycine 2 to alanine
    Promoter
    EF1alpha
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73187-plasmid-map-sequence-id-139537

    Enlarge

  15. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid
    #100941
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
    Tags
    1xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100941-plasmid-map-sequence-id-206116

    Enlarge

  16. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

    Enlarge

  18. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid
    #49098
    Purpose
    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines
    Depositor
    Maxence Nachury
    Publication
    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sstr3 (Sstr3 Mouse)
    Tags
    GFP and acceptor peptide (AP)
    Expression
    Mammalian
    Promoter
    T7
    Available since
    Nov. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49098-plasmid-map-sequence-id-72324

    Enlarge

  20. 5HRE/GFP

    Plasmid
    #46926
    Purpose
    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system
    Depositor
    Martin Brown, Thomas Foster
    Publication
    Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5X HRE of VEGF (VEGFA Human)
    Tags
    d2EGFP
    Expression
    Mammalian
    Mutation
    five copies of a 35-bp fragment from the hypoxia-…
    Promoter
    EF-1α promoter
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46926-plasmid-map-sequence-id-177232

    Enlarge

  23. pEF-XMAS-1xStop

    Plasmid
    #164411
    Purpose
    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.
    Depositor
    Xiao Wang
    Publication
    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164411-plasmid-map-sequence-id-337634

    Enlarge

  26. INCTbiosyn-pEF-GFP(rc)Bxb1

    Plasmid
    #127511
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127511-plasmid-map-sequence-id-250763

    Enlarge

  28. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid
    #127510
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127510-plasmid-map-sequence-id-250757

    Enlarge

  30. pCFJ1415

    Plasmid
    #84828
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84828-plasmid-map-sequence-id-163670

    Enlarge

  31. INCTbiosyn-pEF-GFP(rc)9

    Plasmid
    #127508
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127508-plasmid-map-sequence-id-250750

    Enlarge

  32. INCTbiosyn-pEF-GFP(rc)13

    Plasmid
    #127509
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127509-plasmid-map-sequence-id-250751

    Enlarge

  33. INCTbiosyn-pEF-GFP(rc)2

    Plasmid
    #127504
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127504-plasmid-map-sequence-id-250743

    Enlarge

  34. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

    Enlarge

  35. pEF NS34a-msGFP-'S-mito

    Plasmid
    #158765
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158765-plasmid-map-sequence-id-313128

    Enlarge

  36. INCTbiosyn-pEF-GFP(rc)5

    Plasmid
    #127506
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127506-plasmid-map-sequence-id-250745

    Enlarge

  37. INCTbiosyn-pEF-GFP(rc)7

    Plasmid
    #127507
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127507-plasmid-map-sequence-id-250746

    Enlarge

  38. INCTbiosyn-pEF-GFP(rc)4

    Plasmid
    #127505
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.
    Depositor
    Martin Bonamino
    Publication
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
    Expression
    Mammalian
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127505-plasmid-map-sequence-id-250744

    Enlarge

  39. pEF NS34a-msGFP-'Q-mito

    Plasmid
    #158764
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158764-plasmid-map-sequence-id-312950

    Enlarge

  40. pEF NS34a-msGFP-A'Q-mito

    Plasmid
    #158763
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158763-plasmid-map-sequence-id-312944

    Enlarge

  45. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Publication
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

    Enlarge

  50. pEF1-Pls1-sfGFP

    Plasmid
    #205748
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.
    Depositor
    Jerome Bonnet
    Publication
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls1 (Phyperspank mutant, IPTG inducible)
    Available since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205748-plasmid-map-sequence-id-483889

    Enlarge

Showing: 1 - 50 of 173 results