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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,942 results for: LEA

Showing: 5151 - 5200 of 15942 results

  1. pET21-pelB-LaG-8

    Plasmid
    #172761
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-8
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172761-plasmid-map-sequence-id-340116

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  2. pET21-pelB-LaG-5

    Plasmid
    #172760
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-5
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172760-plasmid-map-sequence-id-340022

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  4. pAAV-CaMKIIa-ChRger3-TS-EYFP

    Plasmid
    #127241
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger3-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127241-plasmid-map-sequence-id-247096

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  9. pICSL11023

    Plasmid
    #117542
    Purpose
    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S_OMEGA(pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117542-plasmid-map-sequence-id-228061

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  11. IBMc097

    Plasmid
    #161945
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161945-plasmid-map-sequence-id-326342

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  12. TR- ATG-HP-GFP

    Plasmid
    #80592
    Purpose
    Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    GFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Inserted 30 nt hairpin which is targeted by Csy4 …
    Promoter
    CBA
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    80592-plasmid-map-sequence-id-151877

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  17. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid
    #127244
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127244-plasmid-map-sequence-id-247231

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  19. pLSU4mg10

    Plasmid
    #126079
    Purpose
    Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Insert
    mitochondria Rieske iron sulphur protein (1-100 aa)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    'long' CaMV35S
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126079-plasmid-map-sequence-id-254915

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  31. pET21-pelB-LaG-2

    Plasmid
    #172739
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-2
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172739-plasmid-map-sequence-id-340192

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  32. pOCC6

    Plasmid
    #118864
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, EGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118864-plasmid-map-sequence-id-232291

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  36. pOCC81

    Plasmid
    #118900
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GST
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease; GST tag
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118900-plasmid-map-sequence-id-232307

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  39. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

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  40. TR-5'UTR-HP-GFP

    Plasmid
    #80594
    Purpose
    Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    GFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Inserted 30 nt hairpin which is targeted by Csy4 …
    Promoter
    CBA
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    80594-plasmid-map-sequence-id-152065

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  41. pMU103

    Plasmid
    #73934
    Purpose
    This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.
    Depositor
    Zhanyuan Zhang
    Article
    Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d
    Insert
    bar and GUS cassettes
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    73934-plasmid-map-sequence-id-139933

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  43. pEPOR1CB0112

    Plasmid
    #117548
    Purpose
    Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117548-plasmid-map-sequence-id-228086

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Showing: 5151 - 5200 of 15942 results