We narrowed to 15,064 results for: pET
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Bacterial Strain#52934PurposeMG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + Δhfq_KanRDepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only
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HL 1179
Bacterial Strain#52935PurposeMG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + Δhfq_KanRDepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7
Plasmid#89458Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTP170 (pBAD33_CRISPR(Tn6900)-entry)
Plasmid#164263PurposeEntry vector for cloning user-defined spacers in the Tn6900 CRISPR array.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterArabinoseAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA
Plasmid#89460Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA
Plasmid#89459Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTP112 (pMS26_miniTn6900(KanR))
Plasmid#164260PurposeDelivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.DepositorInsertminiTn6900 element
UseBacterial delivery vector for tn7 att siteTagsExpressionMutationPromoterAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1054
Plasmid#119241Purposeconstruction intermediateDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1050
Plasmid#119240Purposeconstruction intermediateDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_6xH
Plasmid#186461PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSUN2
Plasmid#188059PurposeBacterial expression plasmid for human NSUN2; for activity testingDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
OPEN-A*24:02
Plasmid#235133PurposeE. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
OPEN-HLA-A*01:01
Plasmid#235134PurposeE. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulinDepositorAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, D40G)-Strep
Plasmid#196210PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationD40GPromoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G38R)-Strep
Plasmid#196209PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG38RPromoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huLbCpf1
Plasmid#90096PurposeBacterial expression plasmid for protein purificationDepositorInserthuLbCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G175R)-Strep
Plasmid#196211PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG175RPromoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G189E)-Strep
Plasmid#196212PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG189EPromoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7
Plasmid#104629Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variationDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b
Plasmid#182276PurposeExpresses recombinant BrCas12bDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis, T7, Thrombin siteExpressionBacterialMutationPromoterT7Available SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
His10-CD3z-SNAPf-ybbR (XSB507)
Plasmid#78508PurposeConstruct for expressing CD3z-SNAPf-ybbR in E. ColiDepositorInsertCD3z (Cytoplasmic domain) (CD247 Human)
UseTagsHis10 and SNAPf-ybbRExpressionBacterialMutationPromoterAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-GIV-CTs
Plasmid#69864PurposeExpress His tagged GIV-CTs (1660–1870).DepositorInsertGIV/Girdin (CCDC88A Human)
UseTagsHisExpressionBacterialMutationC terminal amino acids S1660-S1870 of human GIV.PromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
NSUN6
Plasmid#188060PurposeBacterial expression plasmid for human NSUN6; for activity testingDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK24_HUMAN_D0
Plasmid#79742PurposeThis plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MEF2A-Sensor
Plasmid#208928Purposein vitro MAPK-Sensor for luminescent detection of ERK and p38-activityDepositorInsertMef2a docking sequence+Phospho site (MEF2A Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Trigger_27B
Plasmid#75008PurposePortion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequenceDepositorInsertZIKV_Trigger_27B
UseTagsExpressionBacterialMutationPromoterT7Available SinceFeb. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Trigger_32B
Plasmid#75009PurposePortion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequenceDepositorInsertZIKV_Trigger_32B
UseTagsExpressionBacterialMutationPromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDK2_HUMAN_D0
Plasmid#79726PurposeThis plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYRK2_HUMAN_D0
Plasmid#79729PurposeThis plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK16_HUMAN_D0
Plasmid#79701PurposeThis plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_32B
Plasmid#75011PurposePortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_32B
UseTagsExpressionBacterialMutationPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_27B
Plasmid#75010PurposePortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_27B
UseTagsExpressionBacterialMutationPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA-G83I
Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CSK_HUMAN_D0
Plasmid#79683PurposeThis plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Nsp3(1-111)
Plasmid#242947PurposeGFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)DepositorAvailable SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 UBL-12
Plasmid#227230PurposeExpresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationaa 535-775PromoterT7-promoterAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-His-EWSR1-359-549
Plasmid#195868PurposeExpresses the RRM-RG2-ZnF (359-549) of EWSDepositorAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG086
Plasmid#214263PurposePaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)DepositorInsertHybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
UseTags6x His-tagExpressionBacterialMutationsubstitution of 31 amino acids of FtsH2 (F124RRFA…PromoterT7 promoterAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB120
Plasmid#222213PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tagDepositorInsertfghA(K10N, S11R, W15C, G76V, G258D)
UseTags6x HisExpressionBacterialMutationContains five amino acid mutations: K10N, S11R, W…PromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB143
Plasmid#222214PurposeFor recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tagDepositorInsertvanB(A24P)
UseTagsN-terminal, thrombin-cleavable 6x His tagExpressionBacterialMutationChanged Alanine 24 to ProlinePromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB111
Plasmid#222210PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tagDepositorInsertfghA(G76V)
UseTags6x HisExpressionBacterialMutationChanged glycine 76 to valinePromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB112
Plasmid#222211PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tagDepositorInsertfghA(S11R)
UseTags6x HisExpressionBacterialMutationChanged Serine 11 to ArgininePromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only