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Showing: 5601 - 5650 of 7992 results

  7. TFORF1233

    Plasmid
    #144094
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    AFF2 (AFF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144094-plasmid-map-sequence-id-396168

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  8. TFORF1235

    Plasmid
    #144095
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    AFF2 (AFF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144095-plasmid-map-sequence-id-396164

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  9. TFORF1232

    Plasmid
    #144093
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    AFF2 (AFF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144093-plasmid-map-sequence-id-396161

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  10. pD649-HAsp-SDK1(Trunc3)-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157022
    Purpose
    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SDK1 (SDK1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157022-plasmid-map-sequence-id-303153

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  11. pD649-HAsp-SDK1(Trunc2)-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157009
    Purpose
    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SDK1 (SDK1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157009-plasmid-map-sequence-id-303127

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  12. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid
    #194195
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194195-plasmid-map-sequence-id-381917

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  13. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid
    #194196
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194196-plasmid-map-sequence-id-381925

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  14. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid
    #194197
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 449)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194197-plasmid-map-sequence-id-381946

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  15. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid
    #194191
    Purpose
    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194191-plasmid-map-sequence-id-381776

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  16. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid
    #194192
    Purpose
    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    3 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194192-plasmid-map-sequence-id-381933

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  17. pGL3-pSMANTIS_1kb_KLF2mut 548

    Plasmid
    #194194
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194194-plasmid-map-sequence-id-381930

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  18. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid
    #192835
    Purpose
    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells
    Depositor
    Karin Reinisch
    Article
    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070
    Insert
    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    Use
    Tags
    3xFLAG-PreScission cleavage site and 6xhis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192835-plasmid-map-sequence-id-379957

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  25. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid
    #108289
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108289-plasmid-map-sequence-id-245274

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  26. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid
    #108291
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 192 to A, changed L 195 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108291-plasmid-map-sequence-id-245276

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  31. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  32. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid
    #139997
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139997-plasmid-map-sequence-id-352446

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  33. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  38. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  40. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid
    #71707
    Purpose
    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid
    Depositor
    Giulio Draetta
    Article
    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16
    Insert
    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    Use
    CRISPR
    Tags
    3xFLAG and hGEM(1/110)
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    71707-plasmid-map-sequence-id-424083

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  41. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

    Enlarge

  42. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

    Enlarge

  45. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid
    #229681
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Halo, ALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229681-plasmid-map-sequence-id-457127

    Enlarge

  46. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

    Enlarge

  49. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87421-plasmid-map-sequence-id-441601

    Enlarge

Showing: 5601 - 5650 of 7992 results