We narrowed to 13,452 results for: Mpl;

1. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCMV-intron myc Rab1a S25N

   Plasmid

   #46777

   Purpose

   expression of S25N Rab1a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab1a S25N mutant (RAB1A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N (dominant negative)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pT7-EGFP-C1-HsRCK

   Plasmid

   #25033

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   Rck (DDX6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human MAC-1 alpha

   Plasmid

   #8631

   Depositor

   Timothy Springer

   Article

   Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

   Insert

   MAC-1 alpha (ITGAM Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pTag-RFP-C-h-Rab5a-c-Myc

   Plasmid

   #79802

   Purpose

   Express TagRFP labeled human Rab5a (GTPase located on early endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab5a (RAB5A Human)
   

   Tags

   TagRFP and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. CRISPR-StAR4 (Retro)

   Plasmid

   #222690

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-N-Ezrin

   Plasmid

   #202684

   Purpose

   fluorescent protein tagged ezrin

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   Mus musculus EZR(1-586):EGFP (Ezr Mouse)
   

   Expression

   Mammalian

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CRISPR-StAR4BN (Lenti)

   Plasmid

   #222692

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-n1-APP

   Plasmid

   #69924

   Purpose

   mammalian expression of human APP fused to EGFP

   Depositor

   Thomas Wassmer, Zita Balklava

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Insert

   APP (APP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFRT_TO_FlagHA_G3BP1

    Plasmid

    #127106

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP1 (G3BP1 Human)
    

    Expression

    Mammalian

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. ATG9A-EGFP

    Plasmid

    #210367

    Purpose

    Expresses ATG9A fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pN3-MGA

    Plasmid

    #107715

    Purpose

    Expression plasmid for 3xFLAG-tagged MGA

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Insert

    MGA (MGA Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid

    #160221

    Purpose

    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtACT2::Cas9-nls::tSlATPase
    

    Use

    Synthetic Biology; Moclo toolkit level 1 acceptor…

    Expression

    Plant

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-hMALAT1

    Plasmid

    #118580

    Purpose

    to overexpress human MALAT1 in mammalian cells

    Depositor

    Jianjun Zhao

    Article

    Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

    Insert

    MALAT1 (MALAT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    EF-1a core

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCherry.90 beta

    Plasmid

    #108223

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    mCherry-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-Rab8a-wt

    Plasmid

    #86075

    Purpose

    A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Brm WT-Flag pShuttle

    Plasmid

    #122105

    Purpose

    Expression of full length Brm in mammalian cells

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    BRM (SMARCA2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLIB-HIS-TEV-Cyclin-B

    Plasmid

    #177012

    Purpose

    Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 myc-RNF114

    Plasmid

    #58295

    Purpose

    mammalian expression of myc tagged RNF114

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Tags

    his and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

27. Flag-JunWT-Myc

    Plasmid

    #47443

    Purpose

    mammalian expression of WT c-Jun

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun (Jun Mouse)
    

    Tags

    Flag and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSKA413

    Plasmid

    #80381

    Purpose

    Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    ccaS (complement)
    ccaR (complement)
    gfpmut3
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    FLAG tag

    Expression

    Bacterial

    Promoter

    ccaR and cpcG2Δ59

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-Beclin 1

    Plasmid

    #86748

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Beclin 1 (BECN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTAMAHISTEV_PfeA

    Plasmid

    #128943

    Purpose

    expresses PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDESTmycYBX1

    Plasmid

    #19878

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Y box binding protein 1 (YBX1 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b His-p97

    Plasmid

    #169020

    Purpose

    Expresses His-p97 in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDM1383

    Plasmid

    #207423

    Purpose

    Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

    Depositor

    Rob Kay

    Article

    Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

    Inserts

    HSPC300
    Raf1 (RAF1 Human)
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Mutation

    aa 1–134 (RBD) only

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HA Sec13 pRK5

    Plasmid

    #46332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Sec13 (SEC13 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CRISPR-StAR4B (Lenti)

    Plasmid

    #222691

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCI-HA3-HPS1

    Plasmid

    #133930

    Purpose

    Expresses 3xHA-HPS1 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

    Insert

    HPS1 (HPS1 Human)
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Promoter

    CMV I.E.

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC-GFP-AT

    Plasmid

    #133306

    Purpose

    "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    axe/txe toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTag-BFP-C-h-Rab7L1-c-Myc

    Plasmid

    #79803

    Purpose

    Express TagBFP labeled human Rab7L1.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab7L1 (RAB29 Human)
    

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pTBNde(min)

    Plasmid

    #15125

    Depositor

    Franco Pagani

    Article

    Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

    Insert

    alpha-globin EDB fibronectin minigene (FN1 Human)
    

    Expression

    Mammalian

    Promoter

    alpha-globin

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Mutation

    sgRNA resistant

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMBEC6

    Plasmid

    #187598

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-CAG-DIO-mem-seTurboID

    Plasmid

    #237887

    Purpose

    AAV vector for Cre-dependent expression of membrane-tethered seTurboID

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    mem-seTurboID
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG tag and GAP43 palmitoylation signal

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pT7-V5-SBP-C1-HsGIGYF1_AI

    Plasmid

    #148961

    Purpose

    Mammalian Expression of HsGIGYF1

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF1 (GIGYF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMBP-HRV3C-AcrIF9

    Plasmid

    #141442

    Purpose

    Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

    Depositor

    Blake Wiedenheft

    Article

    Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

    Insert

    AcrIF9
    

    Tags

    HRV3C cleavage site and Maltose Binding Protein

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBRPB CAG-mCherry-IP

    Plasmid

    #106333

    Purpose

    PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    mCherry-IRES-Puro
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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