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We narrowed to 34,850 results for: CaS

Showing: 221 - 240 of 34850 results

  1. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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  4. HypaCas9

    Plasmid
    #138557
    Purpose
    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    HypaCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138557-plasmid-map-sequence-id-274767

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  7. pAcCas12nHS_RV6_empty

    Plasmid
    #203811
    Purpose
    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.
    Depositor
    Quanjiang Ji
    Article
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Insert
    AcCas12nHS_RV6
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203811-plasmid-map-sequence-id-405075

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  9. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49013-plasmid-map-sequence-id-231494

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  11. xCas12i

    Plasmid
    #195333
    Purpose
    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized xCas12i
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195333-plasmid-map-sequence-id-383109

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  12. PB-iCas9

    Plasmid
    #160048
    Purpose
    inducible expression of Cas9
    Depositor
    Xiaojun Lian
    Insert
    Cas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    160048-plasmid-map-sequence-id-313847

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  16. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Article
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109219-plasmid-map-sequence-id-260727

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  20. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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Showing: 221 - 240 of 34850 results