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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,323 results for: Coli

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Showing: 4801 - 4840 of 10323 results

  1. pTar-ipgC

    Plasmid
    #97097
    Purpose
    Expresses ipgC in the presence of E. coli sigma factor ?28
    Depositor
    Richard Murray
    Insert
    IpgC
    Expression
    Bacterial
    Promoter
    pTar
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97097-plasmid-map-sequence-id-182496

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  2. pDest-557

    Plasmid
    #161890
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coli
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    MBP
    Expression
    Bacterial
    Available Since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161890-plasmid-map-sequence-id-322136

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  3. pCDF-mcherry2

    Plasmid
    #87145
    Purpose
    Constitutive E. coli mcherry Expression, with DAS4 tag
    Depositor
    Michael Lynch
    Insert
    mcherry
    Tags
    C terminal DAS4 tag
    Expression
    Bacterial
    Promoter
    Constitutive proD
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87145-plasmid-map-sequence-id-176838

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  4. pAMHDAdGT8-4

    Plasmid
    #26421
    Depositor
    Kohnosuke Mitani
    Insert
    HSV-TK, mCMV-b-gal
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    Jan. 31, 2011
    Availability
    Academic Institutions and Nonprofits only
    26421-plasmid-map-sequence-id-12467

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  5. pOP2S

    Plasmid
    #112597
    Purpose
    Used for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    Strep tag
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112597-plasmid-map-sequence-id-220203

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  6. pCIHDAdGT8-3

    Plasmid
    #26525
    Depositor
    Kohnosuke Mitani
    Insert
    HSV-TK, mCMV-b-gal
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    May 2, 2011
    Availability
    Academic Institutions and Nonprofits only
    26525-plasmid-map-sequence-id-12551

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  7. STK069_pSTK-0D-B0012

    Plasmid
    #235410
    Purpose
    Strong terminator in B. subtilis. Weak transcriptional terminator in E. coli
    Depositor
    Tom Ellis
    Insert
    Bba_B0012
    Expression
    Bacterial
    Mutation
    No
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235410-plasmid-map-sequence-id-502666

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  8. pTar-deGFP

    Plasmid
    #97098
    Purpose
    Expresses GFP in the presence of E. coli sigma factor ?28
    Depositor
    Richard Murray
    Insert
    deGFP
    Expression
    Bacterial
    Promoter
    pTar
    Available Since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97098-plasmid-map-sequence-id-182495

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  11. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182958-plasmid-map-sequence-id-365360

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  13. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Expression
    Bacterial
    Promoter
    Spectinomycin resistance gene promoter and no
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

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  19. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Expression
    Bacterial
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  23. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  27. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available Since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  30. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Available Since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182131-plasmid-map-sequence-id-355782

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  31. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  34. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  38. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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Showing: 4801 - 4840 of 10323 results