We narrowed to 6,993 results for: Cad

1. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199196

   Purpose

   Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAPM-miR30-HNRNPK-ts1

   Plasmid

   #174250

   Purpose

   HNRNPK knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   HNRNPK shRNA (HNRNPK Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM-miR30-HNRNPK-ts3

   Plasmid

   #174252

   Purpose

   HNRNPK knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   HNRNPK shRNA (HNRNPK Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMito-LSSmKate1-N1

   Plasmid

   #31878

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   human cytochrome C oxidase subunit VIII (COX8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   Available since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. phage - ubc - nls - 2xmcp - egfp - BirA*

   Plasmid

   #131132

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Article

   Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

   Insert

   NLS-HA-2xMCP-EGFP-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Chr8q_Centromere-Targeting_gRNA

   Plasmid

   #195128

   Purpose

   gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr8q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEGFP-K140R-STAT3

   Plasmid

   #71448

   Purpose

   Retroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   K140R

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHW581

   Plasmid

   #198818

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAPM-miR30-MAVS-ts2

   Plasmid

   #174275

   Purpose

   MAVS knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MAVS shRNA (MAVS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSP64-hERG1a T623A

    Plasmid

    #53055

    Purpose

    Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Threonine 623 to Alanine

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A mKate2-hu_ncOGT K842M

    Plasmid

    #154284

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    K842M mutation (Catalytic Inactive)

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSP64-hERG1a S624A

    Plasmid

    #53056

    Purpose

    Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Serine 624 to Alanine

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-1 shRev-Erbalpha

    Plasmid

    #22747

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pBItetDT480GFP

    Plasmid

    #96905

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rosa26_gRNA

    Plasmid

    #196138

    Purpose

    gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Rosa26 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBItetDMPKSGFP

    Plasmid

    #96903

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-HA-HA-human CMYC (277-439)

    Plasmid

    #203435

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. J-sRNA-331Bb

    Plasmid

    #78285

    Purpose

    An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    j-sRNA-331
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHW449

    Plasmid

    #198805

    Purpose

    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-PSAT

    Plasmid

    #74115

    Purpose

    Expression of PSAT

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    pET28a-PSAT (PSAT1 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAPM-miR30-DDX58-ts1

    Plasmid

    #174241

    Purpose

    DDX58 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    DDX58 shRNA (RIGI Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLYLQ

    Plasmid

    #80421

    Purpose

    expresses cDNA for human CELF1 (CUGBP1) Flag tagged

    Depositor

    Thomas Cooper

    Article

    Kalsotra et al Proc Natl Acad Sci U S A. 2008 Dec 23;10

    Insert

    human CELF1 cDNA (CELF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEM-human TCR zeta/2470

    Plasmid

    #11507

    Depositor

    Allan Weissman

    Article

    Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

    Insert

    TCR zeta (CD247 Human)
    

    Use

    General cloning

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pGEX5X.1 PINK1 KD

    Plasmid

    #13322

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496; Kinase dead: K219D/D362A/D3…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMH-SFB-DYRK1B

    Plasmid

    #101771

    Purpose

    Mammalian expression of DYRK1B fusion protein

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Insert

    DYRK1B / MIRK (DYRK1B Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

27. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-2TK cyclin T1 (1-300) (P#432)

    Plasmid

    #14608

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-300) (CCNT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-300

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

30. MIGR1-CD5

    Plasmid

    #159329

    Purpose

    Over-expression of mouse CD5

    Depositor

    Nevil Singh

    Article

    Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

    Insert

    CD5 (Cd5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-hPPP3CB-FLAG

    Plasmid

    #179135

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEmpty

    Plasmid

    #214051

    Purpose

    Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. cytosolic moPrP (3F4)

    Plasmid

    #1321

    Depositor

    Susan Lindquist

    Article

    Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

    Insert

    PrP (Prnp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-term truncation (to make gene cytosolic), vario…

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

35. pMito-LSSmKate2-N1

    Plasmid

    #31879

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    human cytochrome C oxidase subunit VIII (COX8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

    Plasmid

    #105643

    Purpose

    Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Article

    Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

37. pBabe puro-HA-PIK3CA(H1047R;I848V)

    Plasmid

    #33253

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and isoleucine…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pBabe HA human Bmf

    Plasmid

    #17239

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf (BMF Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

39. MSCVpuro-6xHis_SUMO2

    Plasmid

    #164938

    Purpose

    Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Small Ubiquitin Like Modifier 2 (SUMO2 Human)
    

    Use

    Retroviral

    Tags

    6XHIS

    Expression

    Mutation

    Promoter

    PGK promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCbs Flag CMYC (bHLH/LZ)

    Plasmid

    #203424

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge