We narrowed to 30,509 results for: Ide

1. pXPR_212

   Plasmid

   #133457

   Purpose

   U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. UNC50-myc

   Plasmid

   #104382

   Purpose

   Expresses UNC50-myc wild-type in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

   Insert

   UNC50 (UNC50 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-51b(+)_NADP-Snifit

   Plasmid

   #113913

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_213

   Plasmid

   #133456

   Purpose

   H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   Control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJFRC7-UAS-NPRR_ANP

   Plasmid

   #133243

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA-backbone

   Plasmid

   #172517

   Purpose

   Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-nls-NgAgo-GK

   Plasmid

   #86435

   Purpose

   Expresses a Flag tag on the N-terminal

   Depositor

   Gaétan Burgio

   Article

   Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

   Insert

   NgAgo
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4-Gateway-SCP1

   Plasmid

   #71510

   Purpose

   humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Humanstarr-seq validation vector

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pRDA_186

   Plasmid

   #133458

   Purpose

   U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET16b-Antp

    Plasmid

    #86866

    Purpose

    Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

    Depositor

    Michael Chin

    Article

    Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

    Insert

    Linker-Antp
    

    Use

    Tags

    Antp

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-2A-2A

    Plasmid

    #74670

    Purpose

    based on pCX-C1 with F2A1/F2A2 inserted

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. enAsCas12a-mSA

    Plasmid

    #182954

    Purpose

    Express enAsCas12a-mSA

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    enAsCas12a-mSA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-beta1,4 GT-1

    Plasmid

    #11872

    Depositor

    Michiko Fukuda

    Article

    Masri et al Biochem Biophys Res Commun. 1988 Dec 15.

    Insert

    beta 1,4 galactosyltransferase (B4GALT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-2T SHP1 CS

    Plasmid

    #8595

    Depositor

    Ben Neel

    Article

    Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

    Insert

    SHP1 (PTPN6 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C453S (catalytically inactive)

    Promoter

    Available since

    June 21, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pET14_GFP5_Encap

    Plasmid

    #86408

    Purpose

    Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 5aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPO640

    Plasmid

    #195296

    Purpose

    pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    miniMcCAST (Tn7575) with KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HsCERK

    Plasmid

    #118600

    Purpose

    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens ceramide kinase
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Feb. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-(RTRG)6

    Plasmid

    #69849

    Purpose

    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

    Insert

    6 RTRG repeats
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJZ001

    Plasmid

    #120230

    Purpose

    Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    intB13 integrase casette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bla-g717a (amp resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Use

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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