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173,387 results

Showing: 5361 - 5400 of 173387 results

  2. pCS2FLAG

    Plasmid
    #16331
    Depositor
    Peter Klein
    Insert
    Flag tag
    Use
    Vertebrate expression
    Tags
    Flag
    Mutation
    Flag tag inserted into pCS2+ polylinker
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
    16331-plasmid-map-sequence-id-316908

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  3. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  5. RedTrackCMV

    Plasmid
    #50957
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
    Depositor
    James Bamburg
    Type
    Empty backbone
    Use
    Adenoviral
    Promoter
    CMV
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50957-plasmid-map-sequence-id-76710

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  8. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

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  9. pYJA5

    Plasmid
    #217778
    Purpose
    The empty vector for quadruple sgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Puromycin resistance gene, T2A, and TagBFP
    Expression
    Mammalian
    Promoter
    PGK, CMV, U6
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    217778-plasmid-map-sequence-id-464940

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  15. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (AAV5)

    Viral Prep
    #50472-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (#50472). In addition to the viral particles, you will also receive purified pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine plasmid DNA. GFAP-driven HA-tagged rM3D(Gs) and bicistronic mCitrine expression, for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    GFAP
    Tags
    mCitrine
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  17. pYPQ203 (pMDC32-Ubi1)

    Plasmid
    #86207
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Expression
    Plant
    Promoter
    Maize ubiquitin 1 (ZmUbi1)
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86207-plasmid-map-sequence-id-174284

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  18. mDsRed-Golgi-7

    Plasmid
    #55832
    Purpose
    Localization: Golgi, Excitation: 557, Emission: 585
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mDsRed
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55832-plasmid-map-sequence-id-494617

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  21. DEXsynth:ccdb-eGFP + RUBY

    Plasmid
    #233317
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb-eGFP + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233317-plasmid-map-sequence-id-464455

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  22. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-TET
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

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  24. TFORF0022

    Plasmid
    #141481
    Purpose
    Lentiviral vector for overexpressing the IRX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IRX3 (IRX3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141481-plasmid-map-sequence-id-276579

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  27. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  30. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid
    #64921
    Purpose
    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPE1 (PCBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64921-plasmid-map-sequence-id-109667

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  31. PermaPhos Kit

    Plasmid Kit
    #1000000226
    Purpose
    Plasmids to express proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) residues in E. coli.
    Depositor
    Ryan Mehl
    Application
    Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  32. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

    Viral Prep
    #107312-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hui Yang
    Article
    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/
    Tags
    mCherry (physically separate, not a fusion protein)
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  40. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available Since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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Showing: 5361 - 5400 of 173387 results