We narrowed to 6,993 results for: Cad

1. pCR-Flag-IKKalpha-KM

   Plasmid

   #15468

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase alpha-KM (Chuk Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Lysine at 44 to Alanine

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pAG109 H2B-FAST

   Plasmid

   #130722

   Purpose

   Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   H2B-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiCrisprV2 sgRNA hPER2c-term #1

   Plasmid

   #179454

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiCrisprV2 sgRNA hPER2c-term #2

   Plasmid

   #179455

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiCrisprV2 sgRNA hPER2c-term #3

   Plasmid

   #179456

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Mouse Alpha2 delta-3 pMT2

   Plasmid

   #58727

   Purpose

   expression of mouse Alpha2 delta-3 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

   Insert

   alpha2 delta-3 (Cacna2d3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR-Flag-IKKbeta-KM

   Plasmid

   #15466

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase beta-KM (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Lysine at 44 to Alanine

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pCJ207

   Plasmid

   #162680

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPU-huMDA5-miR30-MDA5

   Plasmid

   #174224

   Purpose

   All-in-one huMDA5 rescue-MDA5 shRNA knockdown vector

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MDA5 (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDB-hCRY1-mClover3-CD4-bla

    Plasmid

    #179441

    Purpose

    Donor vector to knock in mClover3 C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mClover3
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSicoR Dnmt1

    Plasmid

    #12166

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    Dnmt1 shRNA (Dnmt1 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Promoter

    Available since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Flag-RNF168 delta RING

    Plasmid

    #133982

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of RING

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MSCVhyg-HA-FLAG_Ubc9_WT

    Plasmid

    #164936

    Purpose

    Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    human UBC9 (UBE2I Human)
    

    Use

    Retroviral

    Tags

    FLAG & HA tags

    Expression

    Mutation

    Promoter

    PGK promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Use

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid

    #154293

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations; HCF-…

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEX-YFP-STIM1-deltaK

    Plasmid

    #18861

    Depositor

    Tobias Meyer

    Article

    Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Stop codon inserted at amino acid postion 671 of …

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mouse Alpha2 delta-3 HA pMT2

    Plasmid

    #58728

    Purpose

    expression of HA tagged mouse Alpha2 delta-3 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDB-hCRY1-Halo-CD4-bla

    Plasmid

    #179445

    Purpose

    Donor vector to knock in Halotag C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    HaloTag (Halo7 Synthetic)
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.kc1-HSD17B10

    Plasmid

    #164076

    Purpose

    Human codon optimized HSD17B10. Expression is controlled by doxycycline.

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mApple-proα1(I)

    Plasmid

    #119844

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRRLNeo-pEF1a-p53ashL344P-mCerulean

    Plasmid

    #69581

    Purpose

    Expresses p53-L344P mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents oliogomerization…

    Promoter

    EF1alpha

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV5-FLAG-Gene33

    Plasmid

    #21633

    Depositor

    John Kyriakis

    Article

    Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

    Insert

    Gene33 (Errfi1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114099

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEX-CFP-STIM1-deltaK

    Plasmid

    #18860

    Depositor

    Tobias Meyer

    Article

    Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Stop codon at amino acid position 671 of STIM1. C…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tdEos-LaminB1-10

    Plasmid

    #57632

    Purpose

    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. mcry1K31R HA tag

    Plasmid

    #110297

    Purpose

    mammalian expression of full length mouse CRY1 mutant K31R

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mcry1 K31R (Cry1 Mouse)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    31 Lysines are changed to Argenine

    Promoter

    CMV

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EPHB4-2A-EGFP

    Plasmid

    #139968

    Purpose

    Donor vector to knockin EGFP (separated by 2A) into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    Human EPHB4 knockin homology arms (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tdEos-Mito-7

    Plasmid

    #57644

    Purpose

    Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pcDNA3-Flag-RNF168 delta MIU1

    Plasmid

    #133978

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU1

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pInducer10-SAE1_sh1

    Plasmid

    #168984

    Purpose

    Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MigR1-AEtr

    Plasmid

    #12432

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    C-term truncated AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

32. tdEos-Golgi-7

    Plasmid

    #57619

    Purpose

    Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. mApple-proα2(I)

    Plasmid

    #119840

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114104

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-Flag-RNF168 delta MIU1/MIU2

    Plasmid

    #133981

    Purpose

    mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU1 and MIU2

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pZE21/UBP1/ClpS

    Plasmid

    #98566

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    In a synthetic operon downstream of UBP1 and Remo…

    Promoter

    Same as UBP1 (operon) and Tet promoter (aTc induc…

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mature ISG15 C-term. domain (79-157)

    Plasmid

    #110764

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

    Plasmid

    #196518

    Purpose

    Expression of Ctdnep1_D67E-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67EsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead). Silent mutatio…

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. proISG15 C-term. domain (79-165)

    Plasmid

    #110763

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p029 End-1::EnR

    Plasmid

    #11029

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Dominant inhibitory GATA-binding protein End1::En…

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge