164,811 results

1. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

   Plasmid

   #71485

   Purpose

   Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmtb-t7-alpha-bungarotoxin

   Plasmid

   #69542

   Purpose

   For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

   Depositor

   Sean Megason

   Article

   Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

   Insert

   alpha-bungarotoxin
   

   Use

   Tol2, beta-actin promoter, sp6,t7 for zebrafish

   Tags

   Expression

   Mutation

   Promoter

   beta-actin, sp6, t7

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_EGFP-BRD4-NUT

   Plasmid

   #238254

   Purpose

   For overexpression of EGFP-BRD4-NUT

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EGFP-BRD4-NUT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TagBFP2-CAAX

   Plasmid

   #116856

   Purpose

   plasma membrane marker

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   HRAS
   

   Use

   Tags

   TagBFP2

   Expression

   Mammalian

   Mutation

   Amino acids 172-189

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Wnt7A

   Plasmid

   #35914

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt7A (WNT7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

   Plasmid

   #193851

   Purpose

   E1b promoter for transgenic enhancer assays in zebrafish

   Depositor

   Junsu Kang

   Article

   Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJC5-LAMP1-RFP-3xHA

   Plasmid

   #102932

   Purpose

   Express a lysosomal Tag

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   RFP-HA

   Expression

   Mutation

   Promoter

   ubc

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Puro_hCOL4A4

   Plasmid

   #198099

   Purpose

   lentiviral expression of hCOL4A4 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A4 (COL4A4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

   Plasmid

   #129631

   Purpose

   Visualization of Akt and ERK activity

   Depositor

   Dorus Gadella

   Article

   Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

   Insert

   H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKrox24(2xD-E)dTomato

    Plasmid

    #200110

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. spNW25

    Plasmid

    #173484

    Purpose

    Expresses spNW25 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW25
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p006-Banana-Late

    Plasmid

    #112251

    Purpose

    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    ATF1 under osmY promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    osmY

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol1-UAS:ChrimsonR-tdTomato

    Plasmid

    #124231

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChrimsonR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60

    Plasmid

    #111749

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q60 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-EGFP (AAV2 trial size)

    Viral Prep

    #50465-AAV2.T

    Purpose

    Ready-to-use AAV2 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

16. EYFP-hCALML3

    Plasmid

    #47605

    Depositor

    Emanuel Strehler

    Article

    Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

    Insert

    CALML3 (CALML3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

17. amajLime chromoprotein

    Plasmid

    #117843

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amajLime
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-Empty

    Plasmid

    #91980

    Purpose

    Empty backbone

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMal-T-Avi-His/BirA

    Plasmid

    #102962

    Purpose

    Vector for in vivo biotinylation of a fusion protein in bacteria.

    Depositor

    Tonia Rex

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX311 Luciferase

    Plasmid

    #117735

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Epsin1 deltaUIM-ECFP

    Plasmid

    #20973

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Epsin1 deltaUIM (Epn1 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pEGFP-N1-jGCaMP7b-XC

    Plasmid

    #178361

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP7b.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVITRO1-dV-IgE/κ

    Plasmid

    #61878

    Purpose

    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C1-TBP

    Plasmid

    #26674

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TBP (TBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

    Plasmid

    #60661

    Purpose

    Flp-dependent expression of Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX- Kir2.1-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Epsin1-ECFP

    Plasmid

    #21066

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

31. Human genome-wide lentiviral CRISPR gRNA library version 1

    Pooled Library

    #67989

    Purpose

    Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Species

    Homo sapiens, Mus musculus, and Synthetic

    Use

    CRISPR and Lentiviral

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pGL4.11-Cd36-promoter

    Plasmid

    #138572

    Purpose

    Firefly luciferase reporter fused with a 1296 bp fragment of mouse Cd36 promoter

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 Promoter (Cd36 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBGSA C5aR1-EGFP

    Plasmid

    #62612

    Purpose

    Constitutive expression of C5aR1-EGFP in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Suvorova et al Traffic. 2005 Feb;6(2):100-15.

    Insert

    C5aR1 (C5AR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMXs-Puro-ARHGAP26

    Plasmid

    #69467

    Purpose

    Expresses ARHGAP26

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    ARHGAP26 (ARHGAP26 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. iRFP713-FRB-Fis1tail

    Plasmid

    #220080

    Purpose

    FRB targeted to the mitochondria with the tail of the mitochondrial outer membrane protein Fis1 with an iRFP fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    FRB-Fis1tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-Puro-CLDN18-ARHGAP26

    Plasmid

    #69465

    Purpose

    Expresses Cldn18-Arhgap26 fusion gene.

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    CLDN18-ARHGAP26 (ARHGAP26, CLDN18 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFCyR2

    Plasmid

    #167513

    Purpose

    Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CymRAM-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCymRC

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-NLS-CBGreen-FIRE-puro

    Plasmid

    #139195

    Purpose

    Luminescent reporter of ERK activity

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBG99
    

    Use

    Luciferase and Retroviral

    Tags

    Fra1 PEST domain fragment (FIRE) and nuclear loca…

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-ApoE3

    Plasmid

    #197147

    Purpose

    Expresses ApoE3 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge