264,297 results

1. pSIRV-NF-kB-eCFP

   Plasmid

   #118094

   Purpose

   Flourescent reporter for NF-kB activity

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eCFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NF-kB responsive element

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmRFP-LC3

   Plasmid

   #21075

   Purpose

   Mammalian expression of rat LC3 fused to mRFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-C-terminal GST

   Plasmid

   #128025

   Purpose

   Mammalian expression vector with C terminal GST tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATMW-BL21

   Bacterial Strain

   #218769

   Purpose

   E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

   Bacterial Resistance

   Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

   Species

   Escherichia coli

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pMLS597

   Plasmid

   #188884

   Purpose

   dpy-10 sgRNA expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-TRIM28

   Plasmid

   #124960

   Purpose

   Mammalian expression of Human TRIM28

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-KP1

   Plasmid

   #206504

   Purpose

   AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   KP1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p5

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-MCS2

   Plasmid

   #46954

   Purpose

   Cloning plasmid for the generation of Knock-In in human cells using rAAV.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1/PD-L1

   Plasmid

   #121478

   Purpose

   expression of human PD-L1 in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF155 pcDNA3.1(+)SOD1G93A

    Plasmid

    #26401

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G93A

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LD-B

    Plasmid

    #209861

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CD40L-SrtA

    Plasmid

    #121167

    Purpose

    pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

    Depositor

    Gabriel Victora

    Article

    Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

    Insert

    CD40L-SrtA
    

    Use

    Retroviral

    Tags

    Tomato and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

    Plasmid

    #138315

    Purpose

    Lenti expression of mTgBFP2

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    mTAGBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-mPGK-eGFP (beta-globin intron)

    Plasmid

    #162513

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mPGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW

    Plasmid

    #14883

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-GFP-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFX-mCherry-EEA1

    Plasmid

    #174452

    Purpose

    Express mCherry with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with early endosome-targeting sequence
    

    Use

    Tags

    early endosomal antigen 1 (EEA1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. p2CT-His-MBP-Lbu_C2c2_WT

    Plasmid

    #83482

    Purpose

    Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1/Myc-His(-)-HSulf-2

    Plasmid

    #13004

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 (SULF2 Human)
    

    Use

    Tags

    6x His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. M13KO7-ΔPS-ΔgeneIII

    Plasmid

    #134351

    Purpose

    Helper phage for M13 phage production

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Helper phage gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-Ubiquitin

    Plasmid

    #187910

    Purpose

    Mammalian cell expression of EGFP-tagged Ubiquitin

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac1-6His-DHX9

    Plasmid

    #211440

    Purpose

    generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

    Depositor

    Helmut Pospiech, Frank Grosse

    Article

    Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

    Plasmid

    #226517

    Purpose

    Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    CalR-SNAP-tag2-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AdEasier-1 cells (strain)

    Bacterial Strain

    #16399

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Ampicillin and streptomycin

    Species

    Escherichia coli

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

35. TFORF2908

    Plasmid

    #143663

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIC (NFIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV1)

    Viral Prep

    #83897-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    dTomato

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-X1-Neo-GFP-FAM134B

    Plasmid

    #109026

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmGFP-ADAR1-p150

    Plasmid

    #117927

    Purpose

    ADAR1-p150 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p150 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid

    #67530

    Purpose

    For expression of mCherry fused FRB to the mitochondrial innermembrane

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Inserts

    AIF a.a. 1 - 90
    mCherry
    FRB
    

    Use

    Tags

    AIF 1 - 90, FRB, and mCherry

    Expression

    Mammalian

    Mutation

    T->L

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge