We narrowed to 13,813 results for: cas9 genes

1. pKH-1699

   Plasmid

   #124227

   Purpose

   Mammalian SpCas9-HF1 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS424-ysg(G:H)

   Plasmid

   #138257

   Purpose

   Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   GFP targeting sgRNAs G and H
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-hsg(G:H)

   Plasmid

   #138259

   Purpose

   Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mCherry targeting sgRNAs G and H
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM143

   Plasmid

   #134166

   Purpose

   Retroviral plasmid expressing GFP - Nde1

   Depositor

   Iain Cheeseman

   Article

   Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

   Insert

   Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BII-C3H2B

   Plasmid

   #133387

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gh163

   Plasmid

   #106816

   Purpose

   expression of gRNA targeting TMTC4

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   TMTC4 (TMTC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. gh41

   Plasmid

   #106715

   Purpose

   expression of gRNA targeting ST6GAL2

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ST6GAL2 (ST6GAL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC32

   Plasmid

   #104806

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101090
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC31

   Plasmid

   #104805

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from Gmubi promoter.

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101090
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC27

    Plasmid

    #104801

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr4g020620
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSC26

    Plasmid

    #104800

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from AtUBQ10 promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g101040
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSC20

    Plasmid

    #104794

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from AtUBQ10 promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g020630
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSC14

    Plasmid

    #104788

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g044580
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSC15

    Plasmid

    #104789

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g044580
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSC13

    Plasmid

    #104787

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoter

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr2g044580
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEJS427-pCSDest2-AcrE2

    Plasmid

    #85677

    Purpose

    Mammalian expression of anti-CRISPR protein from Type I-E

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type I-E anti-CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO1-puro-U6-sgRNA-PLXNB2

    Plasmid

    #69240

    Purpose

    U6 driven SpCas9 sgRNA expression for PLXNB2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    PLXNB2 sgRNA (PLXNB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJKW0474

    Plasmid

    #107588

    Purpose

    pCLV3:Cas9

    Depositor

    Jing-Ke Weng

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis CLAVATA3

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-Blasticidin-CAG-Flpe-ERT2

    Plasmid

    #68461

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR_PGK_antiCD19_H1_Gal4VP64

    Plasmid

    #169918

    Purpose

    Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch1-GL4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3015b

    Plasmid

    #209317

    Purpose

    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

    Depositor

    Thomas Hooven

    Article

    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

    Insert

    Group B Streptococcus-compatible single guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Xyl/tet promoter that is constitutively active

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXPR_053

    Plasmid

    #113591

    Purpose

    Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

    Insert

    sgRNA cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. th-P2A-EGFP donor

    Plasmid

    #65562

    Purpose

    Intron targeting-mediated EGFP knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-EGFP (th Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HR-TERT-promotor

    Plasmid

    #71398

    Purpose

    Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. th-P2A-Gal4 donor

    Plasmid

    #65563

    Purpose

    Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-Gal4 (th Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Tol2-LyzC-GFP-U6ac-ctrl-guides

    Plasmid

    #168240

    Purpose

    "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    control sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR_PGK_antiCD19_Z3_Gal4VP64

    Plasmid

    #169916

    Purpose

    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch3-GL4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CSAC-Bmal1

    Plasmid

    #168296

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgBmal1-1-hU6-sgBmal1-3
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. sgLacZs

    Plasmid

    #171185

    Purpose

    Expresses sgRNA

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgLacZ1-hU6-sgLacZ2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid

    #140204

    Purpose

    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Step 1 of cloning c…

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus fumigatus U3 (RNAPIII).

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-BRCA1-RNF169UBD

    Plasmid

    #119009

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge