We narrowed to 18,789 results for: RAN-1

1. pcDNA4/TO-UL87 1-273-2xStrep

   Plasmid

   #138452

   Purpose

   Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   UL87 (UL87 HCMV (HHV-5))
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Amino acids 1-273

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA4/TO-ORF24 1-191-2xStrep

   Plasmid

   #138441

   Purpose

   Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   Amino acids 1-191

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA4/TO-ORF24 1-201-3xFLAG

   Plasmid

   #138420

   Purpose

   Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 1-201 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acids 1-201

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA4/TO-ORF24 1-271-3xFLAG

   Plasmid

   #138421

   Purpose

   Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 1-271 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acids 1-271

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA4/TO-ORF24 1-133-3xFLAG

   Plasmid

   #138422

   Purpose

   Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 1-133 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acids 1-133

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO.1-puro shSlp4-a #2

   Plasmid

   #40070

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   Slp4-a shRNA-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLKO.1 puro shSLC2A1-2

   Plasmid

   #193703

   Purpose

   Constitutive lentiviral expression of SLC2A1 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   SLC2A1 (SLC2A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGL4-Cluster Promoter Delta 1

   Plasmid

   #100126

   Purpose

   It contains ~1.4Kb of the miR-183/96-182 cluster promoter

   Depositor

   Eva Hernando

   Article

   Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

   Insert

   miR-183-96-82 Cluster promoter (MIR182 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid

    #201022

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMKO.1-puro/hFOXH1 shRNA1

    Plasmid

    #59303

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMKO.1-puro/hFOXH1 shRNA3

    Plasmid

    #59308

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGEMHE:mTREK-1(tandem wt-S131C)

    Plasmid

    #224759

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S562C

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEMHE:mTREK-1(tandem S131C-wt)

    Plasmid

    #224758

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T1 Erg (1-208)

    Plasmid

    #66990

    Purpose

    Bacterial expression plasmid for mouse Erg (1-208) with GST

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (1-208) (Erg Mouse)
    

    Use

    Tags

    GST fusion

    Expression

    Bacterial

    Mutation

    Amino acids from 1-208

    Promoter

    TAC

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMKO.1-puro/hFOXH1 shRNA6

    Plasmid

    #59310

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pclbw-opa1(isoform 1)-myc

    Plasmid

    #62845

    Purpose

    Mammalian Expression of myc-tagged Opa1 isoform1

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    mmOPA1isoform1-myc (Opa1 Mouse)
    

    Use

    Retroviral

    Tags

    9xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv-bactin

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-EGFP-AC8(1-106)

    Plasmid

    #181958

    Purpose

    Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    EGFP-AC8(1-106) (Adcy8 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX4T-1 Slp2-a E11A/R32A

    Plasmid

    #40063

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a E11A/R32A (Sytl2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Glutamate 11 to Alanine, Arginine 32 to Alanine

    Promoter

    Tac

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pGEMHE:mTREK-1(tandem S131C-S131C)

    Plasmid

    #224760

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C / S562C

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEMHE:mTREK-1 (S131A/C159A/C219A)

    Plasmid

    #224753

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131A/C159A/C219A

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMHE:mTREK-1 (S131C/C159A/C219A)

    Plasmid

    #224752

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C/C159A/C219A

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. H6Gb1-CREB(1-280) T160C

    Plasmid

    #220233

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    T160C

    Promoter

    T7

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. H6GB1-CREB(1-280) C90A

    Plasmid

    #217982

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    C90A

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX-6P-1-HsTNRC6A_1209-1709_S

    Plasmid

    #147520

    Purpose

    Bacterial Expression of HsTNRC6A_1209-1709

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    HsTNRC6A_1209-1709 (TNRC6A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one non silent mutation K1578E compared to the se…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1 puro shYAP1-2

    Plasmid

    #193693

    Purpose

    Constitutive lentiviral expression of YAP1 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-PU.1-ETS N236H

    Plasmid

    #124616

    Purpose

    Chimeric ETS domain: N236H

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 236 altered from asparagine to a histidi…

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-PU.1-ETS N236Q

    Plasmid

    #124615

    Purpose

    Chimeric ETS domain: N236Q

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 236 altered from asparagine to a glutami…

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-PU.1-ETS N236Y

    Plasmid

    #124614

    Purpose

    Chimeric ETS domain: N236Y

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 236 altered from asparagine to a Tyrosine

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLentiCrisprV2 sgRNA hPER2c-term #1

    Plasmid

    #179454

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human PER2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO.1 puro shCALM2-2

    Plasmid

    #193700

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO.1 puro shCALM2-3

    Plasmid

    #193701

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX2T-TEV CHMP1B 1-143

    Plasmid

    #108286

    Purpose

    Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-143

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28B-PU.1-ETS-H3

    Plasmid

    #124435

    Purpose

    Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Residues 225 to 239 replaced with corresponding s…

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28B-PU.1-ETS-Loop

    Plasmid

    #124551

    Purpose

    Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 216 to 223 replaced with corresponding s…

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28B-PU.1-ETS-S3

    Plasmid

    #124550

    Purpose

    Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 242 to 246 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28b-PU.1-ETS-H2

    Plasmid

    #123358

    Purpose

    Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 207 to 215 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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42. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid

    #196972

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. Rat Alpha2 delta-1 pMT2

    Plasmid

    #58726

    Purpose

    expression of rat alpha2 delta-1 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCEFL GAL4dbd-KLF4 1-329

    Plasmid

    #140146

    Purpose

    GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    KLF4 Kruppel factor (KLF4 Human)
    

    Use

    Tags

    GAL4dbd

    Expression

    Mammalian

    Mutation

    Deleted amino acids 330 to 479

    Promoter

    EF1a

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCEFL GAL4dbd-KLF4 1-156

    Plasmid

    #140147

    Purpose

    GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    KLF4 aa 1-156 (KLF4 Human)
    

    Use

    Tags

    GAL4dbd

    Expression

    Mammalian

    Mutation

    Deleted amino acids 157 to 479

    Promoter

    EF1a

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-GFAP-eGFP-shAqp4-1

    Plasmid

    #223223

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid

    #158792

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon) codon optimized
    

    Use

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. GST-FUS ΔNLS 1-513

    Plasmid

    #44984

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS ΔNLS (1-513aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 1-513

    Promoter

    tac

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

50. pPHR-mCherry-PGL-1-mHP1alpha

    Plasmid

    #181894

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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