167,601 results

1. pcDNA3.1(+)-RIα-EGFP

   Plasmid

   #181840

   Purpose

   GFP-tagged PKA RIα subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-EGFP (PRKAR1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-copGFP-T2A-Puro

   Plasmid

   #72263

   Purpose

   Express copGFP and puromycin resistance gene under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   copGFP-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET22b-mEB1-mCherry-6xHis

   Plasmid

   #130984

   Purpose

   Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB1-mCherry-6xHis (Mapre1 Mouse)
   

   Tags

   mCherry-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-CFP

   Plasmid

   #11179

   Purpose

   Mammalian expression vector for expression of CFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Insert

   ECFP from Aequorea victoria
   

   Expression

   Mammalian

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

   Plasmid

   #85428

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-12
   

   Use

   Lentiviral

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7_CC_PE7_IVT_Template

   Plasmid

   #223022

   Purpose

   Template for in vitro transcription of PE7. For HSPC-related experiments.

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Promoter

   T7

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT-TODestFLAGHA_HuD

   Plasmid

   #65757

   Purpose

   expresses FLAGHA-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-rab11 WT

   Plasmid

   #12674

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. DNMT3A-dCas9

   Plasmid

   #100090

   Purpose

   DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   DNMT3A (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-DNMT3A-dCas9-NLS

   Expression

   Mammalian

   Mutation

   includes aa 602-912

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPSF1

    Plasmid

    #155475

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF1 (CPSF1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christophe Guilhot, Christian Chalut

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

12. pAdTrack-CMV FLAG Rev-Erb alpha

    Plasmid

    #22745

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    Rev Erb alpha (NR1D1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1 NFAT3

    Plasmid

    #10961

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    NFAT3 (NFATC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-Bsd-CMV-MDM4

    Plasmid

    #202669

    Purpose

    MDM4 overexpression vector with blasticidin resistance

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 regulator of p53 (MDM4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ104 - Pmyo-3::mCherry::unc-54

    Plasmid

    #19328

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-cGAS-HA

    Plasmid

    #231895

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. 0_T7-mScarlet3

    Plasmid

    #225927

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWH1266-Apra-sgRNA

    Plasmid

    #218372

    Purpose

    sgRNA expression plasmid for CRISPR-intereference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    sgRNA expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV1)

    Viral Prep

    #20297-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Cre-dependent)

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

20. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. RpH-ILV-3xALFA

    Plasmid

    #225135

    Purpose

    RpH-ILV probe strongly colocalises with lysosomal markers.

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHR-FUSN-mCh-Cry2WT

    Plasmid

    #101223

    Purpose

    FUS(1-214) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

    Plasmid

    #205161

    Purpose

    express β-ACTIN-3′UTR-24xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. GST-AR-1-660

    Plasmid

    #104196

    Purpose

    Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-660 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Available Since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT7-EGFP-C1-HsPatL1_I

    Plasmid

    #146547

    Purpose

    Mammalian Expression of HsPatL1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsPatL1 (PATL1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET_RTX

    Plasmid

    #102787

    Purpose

    Expresses the Reverse Transcriptase gene

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase
    

    Expression

    Bacterial

    Promoter

    T7 promoter + lac operator

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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31. pBBR1k-con-GFPuv

    Plasmid

    #106386

    Purpose

    Constitutive trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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32. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid

    #159872

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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35. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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36. qTAG-N-Blast-mStayGold

    Plasmid

    #227251

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-Wnt10A

    Plasmid

    #35920

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt10A (WNT10A Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-EF1a-Cre

    Plasmid

    #55636

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV11

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100835

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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