167,729 results

1. CMV-Hylight

   Plasmid

   #193447

   Purpose

   A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

   Depositor

   Richard Goodman

   Article

   Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Hylight Biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR223-BcLF1

   Plasmid

   #198581

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BcLF1
   

   Use

   Gateway entry vector

   Mutation

   None (wildtype)

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR223-BXLF2

   Plasmid

   #195830

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BXLF2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BXLF2 (BXLF2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSG5-BBLF4

   Plasmid

   #72634

   Purpose

   Mammalian expression vector for Epstein-Barr virus DNA helicase

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BBLF4
   

   Expression

   Mammalian

   Mutation

   P5L

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. TLS 5:hTLS-CHOP.pCDNA1

   Plasmid

   #21831

   Depositor

   David Ron

   Article

   Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

   Insert

   TLS-CHOP (FUS Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mApple-Centrin2-N-10

   Plasmid

   #54878

   Purpose

   Localization: Centrosomes, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Centrin2 (CETN2 Human)
   

   Tags

   mApple

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUC118-mCtnnb1-FLIP-FlpE-Puro

   Plasmid

   #84541

   Purpose

   CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   mouse beta-catenin FLIP-FlpE - Puro targeting vector
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pLU-CMV-Flag-hSesn2 WT

   Plasmid

   #111776

   Purpose

   Mammalian expression or 3rd generation lentivirus generation of hSesn2

   Depositor

   Jun Hee Lee

   Article

   Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

   Insert

   hSesn2 (SESN2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLC242-GFP-Sestrin2

   Plasmid

   #100519

   Purpose

   Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSG5-BSLF1

    Plasmid

    #69834

    Purpose

    Mammalian expression vector for Epstein-Barr virus primase BSLF1

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BSLF1
    

    Expression

    Mammalian

    Mutation

    S2F

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-RPS6KA5

    Plasmid

    #23758

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RPS6KA5 (RPS6KA5 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. CRISPR-StAR4 (Retro)

    Plasmid

    #222690

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti PGK Hygro DEST (w530-1)

    Plasmid

    #19066

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PPIC

    Plasmid

    #25606

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PPIC (PPIC Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pSG5-BBLF2/3

    Plasmid

    #72633

    Purpose

    Mammalian expression vector for Epstein-Barr virus primase associated factor

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BBLF2/3
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

17. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

19. mito cpVenus control

    Plasmid

    #186792

    Purpose

    Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMTU-DO-HIS-C4

    Plasmid

    #160340

    Purpose

    High-copy expression vector for K. marxianus, histidine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PTK2BA

    Plasmid

    #42401

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTK2BA (PTK2B Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

22. GFP-STONIN2

    Plasmid

    #177885

    Purpose

    Espresses Stonin2 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

    Insert

    stonin2 (STON2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. APP_Halo_C_allele

    Plasmid

    #178137

    Purpose

    Donor vector for endogenous tagging of human APP at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (APP Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMTU-DO-LEU-C4

    Plasmid

    #160341

    Purpose

    High-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TetO-FUW-OSKM

    Plasmid

    #20321

    Purpose

    Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSG5-BALF2

    Plasmid

    #72629

    Purpose

    Mammalian expression vector for Epstein-Barr virus single-strand DNA binding protein

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BALF2
    

    Expression

    Mammalian

    Mutation

    S1028P and V1088A

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

27. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65429

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFD116

    Plasmid

    #124769

    Purpose

    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    dcas9
    PpflB sgRNA BsaI
    

    Use

    CRISPR

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-mPIAS3

    Plasmid

    #15207

    Depositor

    Ke Shuai

    Article

    Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

    Insert

    PIAS3 (Pias3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V580I

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR221_SLC38A10

    Plasmid

    #132083

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMpGE011

    Plasmid

    #71537

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-PTEN

    Plasmid

    #28298

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TTK

    Plasmid

    #38907

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TTK (TTK Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pMC-9-OsAct2-11

    Plasmid

    #197765

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    OsAct2 terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMC-9-ZmUbi-11

    Plasmid

    #197777

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmUbi terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PiggyBac-FLAG-hTET2-IRES-Blast

    Plasmid

    #239819

    Purpose

    PiggyBac vector to express full length human TET2 with 2X N-terminal FLAG tags

    Depositor

    Kasper Rasmussen

    Article

    Knatko et al Blood Adv. 2025 Jun 24:bloodadvances.202

    Insert

    FLAG-tagged human TET2
    

    Tags

    2x FLAG tag on TET2

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge