We narrowed to 17,765 results for: IGH@

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Dendra2-Vimentin-7

Plasmid

#57745

Purpose

Localization: Intermediate Filaments, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vimentin (VIM Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Nano-lantern(cAMP-0.4)/pcDNA3

Plasmid

#53593

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Expression

Mammalian

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtPIF6_X

Plasmid

#131182

Purpose

Expresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPIF6

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-mRuby3

Plasmid

#184898

Purpose

Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

LC3B

Tags

EGFP and mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pId1-IRES-Venus

Plasmid

#22663

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Tags

IRES-Venus

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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mEos2-Golgi-7

Plasmid

#57382

Purpose

Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCS2_RFP-60A

Plasmid

#200394

Purpose

Plasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

RFP

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pC2xTC1-alphaTubulin

Plasmid

#36326

Depositor

Amy Palmer

Article

Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

Insert

alpha tubulin 1b with tetracysteine peptides for ReAsH labeling (TUBA1B Human, Synthetic)

Tags

2xTC1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2012

Availability

Academic Institutions and Nonprofits only

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FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_ScPH1-S

Plasmid

#131193

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
miniSOG-Rab5a-7

Plasmid

#57774

Purpose

Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Rab5a

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMLS716

Plasmid

#188888

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker and Phsp16.41::Cre

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRAB18::LUC

Plasmid

#141168

Purpose

Luminescent reporter for abscisic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtRAB18 promoter

Use

Luciferase

Expression

Plant

Promoter

AtRAB18, AT5G66400

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-CENPA

Plasmid

#57359

Purpose

Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPA (CENPA Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-PTK2-C-10

Plasmid

#55441

Purpose

Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PTK2 (PTK2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
SPI1_pet28a

Plasmid

#131653

Purpose

Bacterial expression of ETS gene SPI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPI1 (SPI1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN331-Kin1B

Plasmid

#31603

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN331 kinesin-1B (Kif5b Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-331

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Vinculin-23

Plasmid

#58030

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vinculin (VCL Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDGE474

Plasmid

#153265

Purpose

M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE475

Plasmid

#153266

Purpose

M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NR6A1_Hormone-recep

Plasmid

#109945

Purpose

Protein expression and purification of NR6A1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR6A1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG Luciferase

Plasmid

#98391

Purpose

Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight and TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CaP2AIII_pK18

Plasmid

#153421

Purpose

Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha constant region (TRAC*02)-P2A

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TET2Puro-gal4DBD-miniVPR-HA

Plasmid

#207169

Purpose

Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-miniVPR

Use

Lentiviral and Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

TRE3G

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-H_peroxisome_C1

Plasmid

#85064

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Abp1-tDimerRFP

Plasmid

#27699

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Abp1 (Dbnl Mouse)

Tags

tDimer-RFP

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Plasmid

#192124

Purpose

pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MCM3-N-22

Plasmid

#54166

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pPDS1-mEGFP-IRES-puro2b

Plasmid

#26464

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pPDS1 (PTTG1 Human)

Tags

mEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-p19-T7-Vif

Plasmid

#46313

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
Vif DNA-1
linker
Vif DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
mRuby2-Cx43-7

Plasmid

#55894

Purpose

Localization: Gap Junctions, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Cx43

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pIAA5::LUC

Plasmid

#141169

Purpose

Luminescent reporter for auxin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtIAA5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtIAA5, At1g15580

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G07

Plasmid

#67141

Purpose

Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRW-CRY2olig-VHH(GFP)

Plasmid

#126023

Purpose

express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Clover-Rab5a-7

Plasmid

#56530

Purpose

Localization: Ras GTPase, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Rab5a

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTubulin-BC2T

Plasmid

#108970

Purpose

expresses tubulin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

a-tubulin

Tags

BC2-tag

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Dendra2-Gamma-Tubulin-17

Plasmid

#57721

Purpose

Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
rtTA and TagBFP cassettes

Plasmid

#62452

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NR4A3_Hormone-recep

Plasmid

#109952

Purpose

Protein expression and purification of NR4A3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR4A3_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RORB_Hormone-recep

Plasmid

#109958

Purpose

Protein expression and purification of RORB_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RORB_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMV::CreERT2-bGHpA

Plasmid

#62443

Purpose

MXS_chaining vector with CMV::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
raACR-pmCerulean3-N1

Plasmid

#204959

Purpose

Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV (+enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PGK-CRY2PHR-VPR

Plasmid

#192608

Purpose

Lentiviral vector for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSZT025

Plasmid

#140033

Purpose

KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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