167,642 results

1. pEF2-EFGR-hMer

   Plasmid

   #225996

   Purpose

   Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

   Depositor

   Sergei Kotenko, Raymond Birge

   Article

   Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

   Insert

   human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)
   

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC-HIS10-ZBASIC-TEV-GG

   Plasmid

   #215797

   Purpose

   Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pNIC-NoTag-GG

   Plasmid

   #215810

   Purpose

   Cloning of target gene with no tags

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. PB-PARP1-del.p119K120S–eGFP

   Plasmid

   #211554

   Purpose

   A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 119K 120S

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-PARP1-delM43;F44I

   Plasmid

   #211579

   Purpose

   CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains deletion of M43 and F44I

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CHFR-PBZ-eGFP

   Plasmid

   #211581

   Purpose

   Expressing CHFR PBZ domain fused to eGFP

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   CHFR PBZ domain (CHFR Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   PBZ domain of the protein

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJR152

   Plasmid

   #196280

   Purpose

   Cassette used in dual sgRNA library generation

   Depositor

   Rebecca Voorhees

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Insert

   hU6-sgRNA-CR3
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL049

   Plasmid

   #198809

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDR111_mannose_Bgal NLS

   Plasmid

   #188398

   Purpose

   Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Promoter

   D-mannose

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-OSF-ATG13 (2-197)

    Plasmid

    #188075

    Purpose

    To make ATG101/ATG13 HORMA complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH6HTC_CCDC124-Halo

    Plasmid

    #175328

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC124 (CCDC124 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCk4

    Plasmid

    #136698

    Purpose

    Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. RV-mCD4-HH5-4

    Plasmid

    #124984

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH5-4 TCRa P2A TCRb
    

    Use

    retroviral

    Expression

    Mammalian

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PDZK1_PDZ_2

    Plasmid

    #103943

    Purpose

    Protein expression and purification of PDZK1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PDZK1_PDZ_3

    Plasmid

    #103944

    Purpose

    Protein expression and purification of PDZK1 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    p10 and pH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAX APP C99

    Plasmid

    #30146

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP C99 (APP Human)
    

    Expression

    Mammalian

    Mutation

    C-terminal from BACE site of APP

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1-MAT2A

    Plasmid

    #100521

    Purpose

    Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    MAT2A (MAT2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTight TRE

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Orf3a-mCherry

    Plasmid

    #165138

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf3a (ORF3a codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Tags

    C9

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. mito-EcSTH

    Plasmid

    #211921

    Purpose

    Mitochondrial localization of EcSTH

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    mito-EcSTH
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV9)

    Viral Prep

    #104492-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

24. pCMV_iPE-C_P2A_Csy4

    Plasmid

    #232428

    Purpose

    Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    iPE-C–P2A–Csy4
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. mCh-GluA1-CIB

    Plasmid

    #89444

    Purpose

    Allows conditional recruitment of AMPA receptors to the postsynaptic membrane

    Depositor

    Matthew Kennedy

    Article

    Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

    Insert

    mCh-GluA1-CIB
    

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRL092 pattB_cryaa:Venus_MCS

    Plasmid

    #213513

    Purpose

    attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDonor14-ShHELIX-KanR (CJT70)

    Plasmid

    #181793

    Purpose

    pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Expression

    Bacterial

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. CAG sHRPa-NRX

    Plasmid

    #73143

    Purpose

    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-NRX
    

    Tags

    2x HA tag and Flag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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