We narrowed to 25,959 results for: Vit;

1. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFH6

   Plasmid

   #86555

   Purpose

   Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   U6-26p::sgRNA scaffold
   

   Use

   CRISPR; Subcloning

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6-26 promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-FRB:Akt2

   Plasmid

   #72899

   Purpose

   Recruitable Akt2 (LocaTOR2)

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   Serine/threonine protein kinase Bβ (AKT2 Human)
   

   Use

   Lentiviral

   Tags

   FRB(T2098L)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-Myo 1d

   Plasmid

   #134906

   Purpose

   Expression of rat Myo 1d (Myr 4) in mammalian cells.

   Depositor

   Martin Bähler

   Article

   Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

   Insert

   Myo 1d (rat) with 3' UTR (Myo1d Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eNme2-C TadCBEa

   Plasmid

   #193840

   Purpose

   Express TadCBEa (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   eNme2-C TadCBEa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFLAG-ING4

   Plasmid

   #13291

   Depositor

   Curtis Harris

   Article

   Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

   Insert

   ING4 (ING4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

7. Hyper7 in pT3TS-Dest

   Plasmid

   #194296

   Purpose

   In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-Stra6L-6His

   Plasmid

   #174215

   Purpose

   Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

   Depositor

   Adrian Press

   Article

   Stafast et al ACS Appl. Polym. Mater. 2022

   Insert

   Stra6L (Stra6l Mouse)
   

   Use

   Tags

   6-His

   Expression

   Mammalian

   Mutation

   non

   Promoter

   CMV

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-SENP5 (catalytic domain)

   Plasmid

   #16358

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP5 catalytic domain (SENP5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 567 to 755 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pFastBac (GST-preSci site-CD45)

    Plasmid

    #177889

    Purpose

    Baculo expression of GST tag fused to human CD45 ICD

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    CD45 (aa 598-1304 only) (PTPRC Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site

    Expression

    Insect

    Mutation

    L652P

    Promoter

    Polyhedrin

    Available since

    Aug. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459-sg-mGreenLantern/EGFP

    Plasmid

    #179913

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCASP-SptP120-K55-HilA

    Plasmid

    #153329

    Purpose

    Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K55 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNAIIIB cdc42 WT

    Plasmid

    #61635

    Purpose

    mammalian expression of cdc42 wt

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    cdc42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Nd1

    Plasmid

    #191523

    Purpose

    Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSynapsin1-axon-GCaMP6m

    Plasmid

    #111261

    Purpose

    For enriched expression of GCaMP6m in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6m
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-evoA1-BE4max

    Plasmid

    #174700

    Purpose

    Base Editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-BE4max
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag Nprl2 pLJM1

    Plasmid

    #46338

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2CT-His-MBP-Lbu_C2c2_R1079A

    Plasmid

    #83494

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1079A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1079A

    Promoter

    T7

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SNF-5 pXOOM

    Plasmid

    #48697

    Purpose

    Expressed SNF-5 in Xenopus oocytes or mammalian cells

    Depositor

    Dmitri Boudko

    Article

    Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

    Insert

    snf-5 (snf-5 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mammalian

    Mutation

    K42E (when compared to NP_496735.1)

    Promoter

    T7

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183605

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFLAG-ING5

    Plasmid

    #13292

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING5 (ING5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pILGFPH7

    Plasmid

    #83554

    Purpose

    Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    ADH2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. N1-mSin1.5-GFP

    Plasmid

    #72909

    Purpose

    GFP-tagged mSin1 (isoform 5)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabe-PI(WT)-Src(KD)-mCherry

    Plasmid

    #87358

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    D388R

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSD117A

    Plasmid

    #218141

    Purpose

    Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD40
    

    Use

    Tags

    AviTag-6xHis-MBP-TEV-(GGGGS)2

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD-HisD-KRaION1

    Plasmid

    #177799

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

    Plasmid

    #136270

    Purpose

    Expresses ABE and ST2-com modified sgRNA scaffold

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Insert

    ABE and sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCB128

    Plasmid

    #205137

    Purpose

    Expression of mCherry-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Use

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

    Plasmid

    #112006

    Purpose

    For co-expression of GCaMP6s with mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-hSyn-Gαi*-BERKY3

    Plasmid

    #158428

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSIREN-RetroQ-shLuc

    Plasmid

    #73666

    Purpose

    pSRIEN-RetroQ vector containing control sequence luciferase

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCMV-FKBP-mTagBFP2-SOScat

    Plasmid

    #178866

    Purpose

    Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-mCherry-TSC2-NLS

    Plasmid

    #192441

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMAL-PUM2

    Plasmid

    #120385

    Purpose

    Bacterial expression of MBP-Pumillio2 fusion protein

    Depositor

    Igor Ulitsky

    Article

    Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

    Insert

    PUM2 (PUM2 Human)
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge