We narrowed to 4,430 results for: Erf

1. pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

   Plasmid

   #233067

   Purpose

   Piggybac CRISPRi construct

   Depositor

   Markus Landthaler

   Article

   Vučićević et al bioRxiv 2024.09.12.612198

   Insert

   KRAB-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLD-puro-Cc-IFIT5_K197R-VA

   Plasmid

   #191223

   Purpose

   Lentiviral expression of human IFIT5_K197R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 197 to Arginine (K197R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD-puro-Cc-IFIT5_K160R-VA

   Plasmid

   #191222

   Purpose

   Lentiviral expression of human IFIT5_K160R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 160 to Arginine (K160R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG0A0-1-1-PBAD-dCas9

   Plasmid

   #208815

   Purpose

   Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

   Depositor

   Marc Erhardt

   Article

   Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

   Insert

   Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinsoe-inducible promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+-Zeo-miR-430

   Plasmid

   #221726

   Purpose

   Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   BleoR-miR-430
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   simian CMV IE94

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-hfYFP-TEV-Bcx

   Plasmid

   #191195

   Purpose

   Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Bcx fusion
   

   Use

   Tags

   6xHis and Bacillus circulans xylanase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLei-sfGFP-V2*D134*Y152*

   Plasmid

   #191112

   Purpose

   An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing V2 & D134 & Y152 to TAG

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_mcherry

   Plasmid

   #167870

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pVH321-1-Tier1-PhCMV-dCas9-3xNLS

   Plasmid

   #169595

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-NF1-L1-dSpRY

    Plasmid

    #177681

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ-NF1-L2-dSpRY

    Plasmid

    #177682

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_iRFP713

    Plasmid

    #167868

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_m4_Hygro

    Plasmid

    #167867

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. NAX_CAGGS_CAS9_m4_EBFP2

    Plasmid

    #167865

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p415:iCas9

    Plasmid

    #138266

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Cas9-D10A, H840A

    Promoter

    GalL

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK3b_030

    Plasmid

    #123808

    Purpose

    Encodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NLS::dCAS9::NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0.018

    Plasmid

    #119622

    Purpose

    Level 0 Part. CDS

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    dCas9+deg-tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJUMP18-dCas9_O

    Plasmid

    #127028

    Purpose

    Basic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part dCas9_O
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL-RNAINS4-C24A

    Plasmid

    #69462

    Purpose

    RNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 with C24A mutation
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    C24A and mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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