We narrowed to 14,109 results for: Cre
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Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorInsertVP64dCas9VP64-2A-MPH
UseCRISPRAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)
Plasmid#169161PurposeTo express nsp10-nsp14 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)
Plasmid#203908PurposeEncodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cellsDepositorInsertGal4UAS_dGFP_PGK_iRFP
ExpressionMammalianPromoter5xUAS and PGKAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-6.20
Plasmid#41002DepositorInsertGSG-P2A-RLuc
UseLuciferaseExpressionMammalianPromoterSV40Available SinceNov. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Bhlhe40-3xFLAG-IRES-BFP
Plasmid#117263PurposeRetroviral overexpression of Bhlhe40 with a 3xFLAGDepositorAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.18 NR0B1-Luc
Plasmid#100983PurposeNR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoterDepositorInsertNR0B1 (NR0B1 Human)
ExpressionMammalianAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.1-Fc-His
Plasmid#72098PurposeExpresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/empty
Plasmid#105505PurposeEmpty backbone of MSCV-driven retroviral cDNA expressionDepositorTypeEmpty backboneUseRetroviralAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
VSVG-YFP-3xUVR8
Plasmid#49801Purposeexpresses VSVG fused to UVR8 for light-triggered traffickingDepositorInsertVSVG-YFP-3xUVR8
TagsHA, MYC, and YFPExpressionMammalianMutationVSVG contains ts045 mutationsAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-mCherry
Plasmid#65259Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsStreptavidin Binding Protein (SBP) and mCherryMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiAsCpf1-3xMYC-blast
Plasmid#127750PurposeLentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signalDepositorInsertAsCpf1
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKL123
Plasmid#187889PurposeAAVS1 safe-harbor integration of dox-inducible NFIA-SOX9DepositorInsertNFIA-SOX9
ExpressionMammalianPromoterTRE3GAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETNb-nALFA
Plasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector
Plasmid#99292PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorExpressionMammalianAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pstb-LAFR5
Plasmid#86169PurposepLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.DepositorTypeEmpty backboneExpressionBacterialPromoterSMb21651 promoterAvailable SinceFeb. 6, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNH082
Plasmid#42165DepositorInsertmTFP1
ExpressionWormAvailable SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry_Atg4BC74A
Plasmid#161735PurposeDoxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagyDepositorInsertAtg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsmStrawberryExpressionMammalianMutation220-222 TGC (Cys) is changed to GCA (Ala)Available SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
ExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
nls-transposase-nls-pA in pT3TS-Dest
Plasmid#140873PurposeVector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.DepositorInsertnls-transposase-nls (Tol2)
Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
CD4d3+4-blac
Plasmid#32403DepositorTypeEmpty backboneTagsbeta-lactamase, pentamerization domain of rat car…ExpressionMammalianPromoterCMV promoterAvailable SinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPINVH
Plasmid#26041DepositorInsertu-LacZ-KHHHHHH
TagsKHHHHHH and u-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
Plasmid#52890Purpose"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.DepositorInsertsExpressionInsectAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA (long) wt
Plasmid#19717DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralExpressionMammalianMutationNOTE: long splice variant with 3bp deletion and 1…Available SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-FRT hM4Di-mCherry
Plasmid#161576PurposeExpression of hM4Di-mCherry in a Flp-dependent fashionDepositorInserthM4Di-mCherry
UseAAVMutationNAPromoterhSynAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-UFM1-dCS
Plasmid#139869PurposeMammalian expression of HA-tagged human UFM1-cCSDepositorAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF1_P2A_Hygro_Barcode
Plasmid#120488PurposeBarcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BRAT1
Plasmid#62802PurposeExpresses human BRAT1 with an N-terminal HA tag in mammalian cellsDepositorAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCL_P2A_Hygro_Barcode
Plasmid#120462PurposeBarcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH6
Plasmid#86555PurposeSubcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiencyDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR; SubcloningPromoterArabidopsis U6-26 promoterAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only