We narrowed to 24,746 results for: CHI

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pET15b T7pCONS TIR-2 sfGFP

Plasmid

#154468

Purpose

pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-kanMX6

Plasmid

#39294

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-TMCC2

Plasmid

#121047

Purpose

Expresses human TMCC2 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 2 (TMCC2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHSE901

Plasmid

#91708

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-nlsCRE

Plasmid

#12265

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Promoter

unknown

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSL4393 (pClpX_Eco)

Plasmid

#200907

Purpose

Expresses human codon-optimized E. coli ClpX from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

EcoClpX

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-deltaBamHI-human MIZ1

Plasmid

#74167

Purpose

Mammalian expression of human MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE-EGFP_IRES-Blast

Plasmid

#213113

Purpose

Expression of MinE-EGFP in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE-EGFP (minE Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAurora

Plasmid

#190158

Purpose

Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

Depositor

Andreas Moeglich

Article

Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

Type

Empty backbone

Tags

His6 tag

Expression

Bacterial

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S94A

Plasmid

#33102

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S94A

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-TEAD4

Plasmid

#33105

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD4 (TEAD4 Human)

Tags

Gal4

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pET-NtLNtC60αC20C60β

Plasmid

#160874

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: pBAD, chaperones: T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTF-lacZ-rfp

Plasmid

#153037

Purpose

CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-MinD-IRES-Puro

Plasmid

#213114

Purpose

Expression of untagged MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-myrlysin/BORCS5

Plasmid

#194332

Purpose

Expresses myrlysin in mammalian cells

Depositor

Juan Bonifacino

Article

Pu et al Dev Cell. 2015 Apr 20;33(2):176-88. doi:

Insert

BORCS5 (BORCS5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL002

Plasmid

#98921

Purpose

pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Inserts

PROPS
GCaMP6f

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL-Blast-Flag-HA-hTERT

Plasmid

#22396

Depositor

William Hahn

Article

Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

Insert

TERT (TERT Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126218

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

Lentiviral

Expression

Mammalian

Promoter

CamKIIa

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

Plasmid

#74317

Purpose

A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

Depositor

Martin Meyer, Stephen Smith

Article

Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Inserts

synaptophysin
DsRedExpress

Tags

EGFP

Promoter

5UAS

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGF_RT_FAST_RFP

Plasmid

#136979

Purpose

Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

Depositor

Klaus Theres

Article

Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

Insert

seed coat marker RFP

Expression

Bacterial

Mutation

BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXSE901BG

Plasmid

#91714

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCSUP35(1-253)

Plasmid

#1089

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

amino acids 1 - 253 of SUP35

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
CMV-p150

Plasmid

#51412

Purpose

expression of full length of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt7 shRNA1

Plasmid

#53144

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a T7pCONS TIR-1 sfGFP

Plasmid

#154463

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV4-p100

Plasmid

#23287

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

NFkB p100 subunit (NFKB2 Human)

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.771

Plasmid

#108534

Purpose

NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

NahRAM + PSalTTC-YFP

Expression

Bacterial

Mutation

NahR: I25V P72H V118A R248C

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Gdf6

Plasmid

#163598

Purpose

Expresses GDF-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf6 (Gdf6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
plYJP022-int7

Plasmid

#202287

Purpose

Plasmid containing integrase for LPattB7 site

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

int7

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pScalps_Puro

Plasmid

#99636

Purpose

Empty backbone, 3rd generation lentiviral vector

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

SFFVp

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-UFM1

Plasmid

#134640

Purpose

Expression of FLAG-tagged human UFM1 wildtype

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZmUBQ:GW

Plasmid

#132358

Purpose

Expression of CDS from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Type

Empty backbone

Tags

YFP

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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