We narrowed to 6,994 results for: Cad

1. His-FLAG-Tev_ACTA1 (alpha-actin)

   Plasmid

   #188452

   Purpose

   Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   ACTA1 (ACTA1 Human)
   

   Use

   Tags

   6xHis-FLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p5E-1.7kbfabp6

   Plasmid

   #159087

   Purpose

   p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Insert

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   

   Use

   Promoter fragment 5' entry vector for tol2 c…

   Tags

   Expression

   Mutation

   Promoter

   1.7kb fabp6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXL003-ishRNA-beta-catenin-2

   Plasmid

   #36299

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-2 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pFRLuc-CoV2-FLucStop

   Plasmid

   #177617

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   In-frame stop codon added between FLuc and FSE

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-TetOne-Puro-PFDN2

   Plasmid

   #202664

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   prefoldin subunit 2 (PFDN2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLJM1-PHD2

   Plasmid

   #223552

   Purpose

   Lentivirus transfer plasmid for expression of full length human PHD2

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMH-SFB-USP7

   Plasmid

   #99393

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   USP7 (USP7 Human)
   

   Use

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRES2-DsRed-MST-Kv2.1

   Plasmid

   #131707

   Purpose

   Expresses Kv2.1in mammalian cells

   Depositor

   Jennifer Kearney

   Article

   Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

   Insert

   KCNB1 (KCNB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pDB-hPER2-mScarlet-I-CD4-bla

    Plasmid

    #179449

    Purpose

    Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mScarletI
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-mTurquoise-MLC-IRES-neo

    Plasmid

    #85145

    Purpose

    lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mTurquoise

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a-VHH-2xTS

    Plasmid

    #109419

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1017 pcDNA3 Myr Akt1 deltaPH

    Plasmid

    #9009

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 dPH (AKT1 Human)
    

    Use

    Tags

    myr (MGSSKSKPKDPSQR)

    Expression

    Mammalian

    Mutation

    delta PH domain (aa1-130)

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-HemmarG

    Plasmid

    #31221

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Insert

    CD4-tdGFP (CD4 Human, Fly, Aequorea victoria)
    

    Use

    Tags

    CD4 and EGFP/GFP

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His6tev CBP BCHZ (1079-1751)

    Plasmid

    #99341

    Purpose

    Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
    

    Use

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 MLK1 E179K

    Plasmid

    #60541

    Purpose

    Donor vector for human MLK1 E179K mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    E179K

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDONR221 MLK1 D294A

    Plasmid

    #60542

    Purpose

    Donor vector for human MLK1 D294A mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    D294A

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV14_NHP2L1(15.5kD)

    Plasmid

    #73065

    Purpose

    expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    NHP2L1 (15.5kD) (SNU13 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe DN-FOXO1-HA neo

    Plasmid

    #45814

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1-255

    Promoter

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid

    #100282

    Purpose

    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-621 aa)

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA Flag PGC1 T177A S538A

    Plasmid

    #18093

    Depositor

    Bruce Spiegelman

    Article

    Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T177A S538A

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pAPM-miR30-EIF2AK2-ts1

    Plasmid

    #174247

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCR-HA-IKKalpha

    Plasmid

    #15469

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

26. Met1 diUb (1-152)

    Plasmid

    #110766

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR201-Eps8L2

    Plasmid

    #196526

    Purpose

    Entry vector for Gateway with Eps8L2

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2-FL (EPS8L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-ZIP(WT)

    Plasmid

    #27134

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGPN-ACE2

    Plasmid

    #187483

    Purpose

    Expression vector - ACE2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLINK cyclin T1 (1-280) myc (K93D) (P#580)

    Plasmid

    #14611

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) K93D (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K93D. Deficient in binding to CDK9. Inactive in 3…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only