We narrowed to 11,256 results for: NSI
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pHH0103_MIA3-1/1
Plasmid#91327PurposeProtein expression and purification of human SH3 domain construct MIA3-1/1DepositorInsertMIA3-1/1 (MIA3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PLCG1-1/1
Plasmid#91540PurposeProtein expression and purification of human SH3 domain construct PLCG1-1/1DepositorInsertPLCG1-1/1 (PLCG1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGL3-64/+19
Plasmid#35543DepositorUseLuciferaseTagsluciferaseExpressionMutationPromoternoneAvailable sinceAug. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NDUFS6(5’UTR)-FF
Plasmid#85488PurposeFirefly luciferase under the control of NDUFS6 5'UTRDepositorInsert5'UTR of NDUFS6 (NDUFS6 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD10AH840AhCas9 (GB1041)
Plasmid#68207PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid partDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removed; human codon optimis…PromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(84Q)-S776A
Plasmid#21758DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 84 Q(Glutamines) and…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(30Q)-S776A
Plasmid#21756DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 32 amino acids (30 Q…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3b(1454/3172)P2P-mut
Plasmid#21405DepositorInsertEGLN1 (EGLN1 Human)
UseLuciferaseTagsExpressionMammalianMutationhypoxia response element mutatedPromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-I560S-mDPYD
Plasmid#61615PurposeThe mouse Dpyd was used to rescue the human DPYD hairpin affect on EMTDepositorInsertDihydropyrimidine dehydrogenase (Dpyd Mouse)
UseTagsExpressionMammalianMutationChanged Isoleucine at position 560 to SerinePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1-PIP3A
Plasmid#37142DepositorInsertE2F1-P!P3A (E2f1 Fly)
UseGatewayTagsExpressionMutationI153, Y156, Y157 to AlaninePromoterAvailable sinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
Tg(Sox17::EMTB-3xGFP)
Plasmid#221190PurposeTo specifically label microtubules (MTs) in Kupffer's Vesicle (KV)DepositorInsertEnsconsin Microtubule-Binding Protein (MAP7 Human)
UseZebrafishTags3 EGFPExpressionMutationMicrotubule binding-domain of ensconsin (A.A. 18…PromoterSox17Available sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_E307K
Plasmid#234560PurposeCTNNA1 butterfly eye mutantDepositorInsertmEGFP:hCTNNA1_E307K (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationE307KPromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_R551A
Plasmid#234558PurposeCTNNA1 M2-M3 salt-bridge disrupting mutantDepositorInsertmEGFP:hCTNNA1_R551A (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationR551APromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM2,3-domain
Plasmid#234556PurposeCTNNA1 M2,3-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa 397-509PromoterCMVAvailable sinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM1-domain
Plasmid#234555PurposeCTNNA1 M1-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa 271-396PromoterCMVAvailable sinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)
Plasmid#238189PurposeLentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertPLAGL1 sgRNA CRISPRa (PLAGL1 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)
Plasmid#238169PurposeLentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertCD45 sgRNA CRISPRa (PTPRC Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-CKAR2
Plasmid#236100PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.DepositorInsertLAMP1-ExRai-CKAR2 (LAMP1 Synthetic, Human)
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Nluc-_(PLRSKK)-CA1-19
Plasmid#232657PurposeNluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assayDepositorInsertCGA (CGA Human)
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-HLA-A0201-ST3
Plasmid#223220PurposeExpression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteriaDepositorInsertHLA--A2 heavy chain (HLA-A Human)
UseTagsSpytagExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2T-U6-sgCTG
Plasmid#232723PurposeSpCas9 guide RNA targeting CAG repeats for C-to-T base editingDepositorInsertSpCas9 gRNA targeting CAG repeats
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228373PurposeExpresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana AsnRS evm.model.fragScaff scaffold 419.110 (SHI02794)
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228377PurposeExpresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228379PurposeExpresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana ProRS evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA)
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Athal_PheRS_TP_pK7fWG2_IVD_FP611
Plasmid#228370PurposeExpresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371DepositorInsertN-terminal transit peptide from Arabidopsis PheRS At3g58140 (Warren et al., 2023) with demonstrated dual mito/plastid targeting.
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC8
Plasmid#224343PurposeExpresses human KDAC8 (HDAC8) in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationPromoterPolyhedrinAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A
Plasmid#222855PurposeMammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn bindingDepositorInsertAT118-L
UseTagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329G, H435A, I253A, H310APromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
LCV2 PQBP1 KO2
Plasmid#217448PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1DepositorInsertsgRNA targeting PQBP1 (PQBP1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA Q63/67A
Plasmid#217439PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA Q63A,Q67APromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA Q67H/N74D
Plasmid#217441PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA Q67H,N74DPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
LCV2 PQBP1 KO1
Plasmid#217447PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1DepositorInsertsgRNA targeting PQBP1 (PQBP1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-SS-VC155-IRE1-dLD
Plasmid#217751PurposeExpresses the VC fragment of BiFC-IRE1-dLDDepositorInsertVC155-IRE1-dLD (ERN1 Human)
UseTagsVenus (155-238, A206K)ExpressionMammalianMutationdeleted amino acids 29-408PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-WIPI1promotor
Plasmid#206840PurposeThe plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).DepositorInsertWIPI1 promotor (partial)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pINQ-ON10
Plasmid#198689PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, HA tag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINQ-NbH4
Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
UseTags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP6 145-201
Plasmid#104597PurposeExpresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11DepositorInsertCHMP6 residues 145-201 (CHMP6 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP7 366-453
Plasmid#104598PurposeExpresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12DepositorInsertCHMP7 residues 366-453 (CHMP7 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only
