167,785 results

1. pPB-CAG-ARID1A-deltaIDR1-FLAG

   Plasmid

   #242210

   Purpose

   Expression of human ARID1A ∆IDR1 (deletion of a.a. 2–970) using piggyBac transposition

   Depositor

   Courtney Hodges

   Article

   Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Piggybac

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted IDR1 from ARID1A (deletion of a.a. 2–970)

   Promoter

   CAG

   Available Since

   Sept. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-GFP-GCN4-ppKin14

   Plasmid

   #240427

   Purpose

   -end oriented kinesin for rapalog induced pulling experiments.

   Depositor

   Anna Akhmanova

   Article

   Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

   Insert

   ppKin14Vib
   

   Tags

   FRB, GCN4, and GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-850

   Available Since

   July 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-mBaoJin-P2A-mCherry

   Plasmid

   #229601

   Purpose

   Expresses mBaoJin and mCherry in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

   Insert

   mBaoJin
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CaMK2rep

   Plasmid

   #239622

   Purpose

   CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Article

   Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMK2rep-S603A

   Plasmid

   #239623

   Purpose

   Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Article

   Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Mutation

   changed Serine 603 to Alanine

   Promoter

   CMV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. nCaMK2rep

   Plasmid

   #239624

   Purpose

   CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Article

   Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

   Insert

   PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag and myc

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. nCaMK2rep-S603A

   Plasmid

   #239625

   Purpose

   Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Article

   Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

   Insert

   PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Changed serine 603 to Alanine

   Promoter

   hSyn

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW H2B-mEmerald-P2A-PuroR

   Plasmid

   #239553

   Purpose

   Expression and nuclear localization of mEmerald

   Depositor

   Tim Ahfeldt, Joel Blanchard

   Article

   et al iScience 28 (2025)

   Insert

   mEmerald
   

   Use

   Lentiviral

   Promoter

   UbC

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-TR-hSOD1

   Plasmid

   #232478

   Purpose

   Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Homo sapiens superoxide dismutase 1 (SOD1 Human)
   

   Use

   Transposon system

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Promoter

   CMV-TetO

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. HDM_RSV_A2_G_31AACTdel

    Plasmid

    #237352

    Purpose

    Expression plasmid for RSV A2 G

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A2 G human codon optimized from Genbank Accesion KT992094.1
    

    Expression

    Mammalian

    Mutation

    31 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_ATG101

    Plasmid

    #223796

    Purpose

    Expression of recombinant protein for purification (co-expression with ATG13)

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG101 (ATG101 Human)
    

    Expression

    Mammalian

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ-Myc-IRF2

    Plasmid

    #232642

    Purpose

    transient expresses IRF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF2 (IRF2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP15263 - pAAV-AiE0219h-minBG-iCre(R297T)-BGHpA (Alias: CN5263)

    Plasmid

    #230406

    Purpose

    AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBbleo-EREG-ScNeo

    Plasmid

    #209905

    Purpose

    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EREG-ScNeo (EREG Human)
    

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF2-EFGR-hMer

    Plasmid

    #225996

    Purpose

    Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

    Insert

    human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)
    

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNIC-HIS10-ZBASIC-TEV-GG

    Plasmid

    #215797

    Purpose

    Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pNIC-NoTag-GG

    Plasmid

    #215810

    Purpose

    Cloning of target gene with no tags

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. PB-PARP1-del.p119K120S–eGFP

    Plasmid

    #211554

    Purpose

    A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 119K 120S

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CHFR-PBZ-eGFP

    Plasmid

    #211581

    Purpose

    Expressing CHFR PBZ domain fused to eGFP

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    CHFR PBZ domain (CHFR Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    PBZ domain of the protein

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJL049

    Plasmid

    #198809

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDR111_mannose_Bgal NLS

    Plasmid

    #188398

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Promoter

    D-mannose

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAG-OSF-ATG13 (2-197)

    Plasmid

    #188075

    Purpose

    To make ATG101/ATG13 HORMA complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pH6HTC_CCDC124-Halo

    Plasmid

    #175328

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC124 (CCDC124 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCk4

    Plasmid

    #136698

    Purpose

    Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. RV-mCD4-HH5-4

    Plasmid

    #124984

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH5-4 TCRa P2A TCRb
    

    Use

    retroviral

    Expression

    Mammalian

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. PDZK1_PDZ_2

    Plasmid

    #103943

    Purpose

    Protein expression and purification of PDZK1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. PDZK1_PDZ_3

    Plasmid

    #103944

    Purpose

    Protein expression and purification of PDZK1 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    p10 and pH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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