167,433 results
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Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N EBNA1
Plasmid#37954DepositorInsertEBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianPromoterPKGAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP
Plasmid#89684PurposepCAG promoter driving EGFPDepositorInsertEGFP
UseSynthetic BiologyExpressionMammalianAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCh-Rab7A
Plasmid#61804PurposeHuman Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expressionDepositorAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EgI-HygroR
Plasmid#207177PurposeGateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroRDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM1
Plasmid#179955PurposeExpression vector for human IFITM1.DepositorAvailable SinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE26
Plasmid#90368PurposeC/EBPb - CEBPB gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterCEBPbAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon600
Plasmid#126207Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon600
ExpressionMammalianAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
STK11 gRNA (BRDN0001146194)
Plasmid#75913Purpose3rd generation lentiviral gRNA plasmid targeting human STK11DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ERBB2_PPP1R1B
Plasmid#205813PurposeExpress mEGFP-tagged fusion protein, ERBB2_PPP1R1B from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-FOS
Plasmid#192924PurposeBarcoded piggybac transposon vector with Dox-inducible expression of FOSDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e(TadA V106W)
Plasmid#138509PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSaCas9
ExpressionMammalianMutationTadA-8e V106WAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST
Plasmid#196341PurposeOverexpression of circular RhoBASTDepositorInsertTornado-RhoBAST
ExpressionMammalianPromoterU6+27Available SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag
Plasmid#132397Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1058AAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Flag-SENP1
Plasmid#17357DepositorAvailable SinceMarch 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
769 pME mCerulean-CAAX
Plasmid#195957PurposeGateway pME fluorophoreDepositorInsertmCerulean-CAAX (membrane)
UseOtherAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag
Plasmid#132398Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)
Viral Prep#130991-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (#130991). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. CamKIIa-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmScarletAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
ePPE-spG
Plasmid#183096PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nspGCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpGCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-ATG5
Plasmid#13095DepositorAvailable SinceNov. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA1
Plasmid#26677DepositorInsertKaryopherin Alpha 1 (KPNA1 Human)
TagsT7ExpressionMammalianMutationKPNA1 sequence contains a reported SNP at S73NAvailable SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2207
Plasmid#143354PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianPromoterpTRE bidirectional tet-onAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY010 (pcDNA3.1-hAsCpf1)
Plasmid#69982PurposeExpresses humanized AsCpf1DepositorInserthAsCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag Runx1 FL (P#1790)
Plasmid#14585DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) (AAV PHP.eB)
Viral Prep#163509-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) (#163509). In addition to the viral particles, you will also receive purified AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) plasmid DNA. hSyn1-driven expression for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsSYFP2Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-FLuc
Plasmid#188311PurposeExpression of Firefly luciferase via a T7 promoter in TXTLDepositorInsertFirefly luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialPromoterT7MaxAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBE45
Plasmid#174655PurposeCAPTURE method receiver plasmid for Streptomyces heterologous expressionDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔF
Bacterial Strain#102259PurposeompF deletion strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceNoneAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianPromoterT7 PromoterAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KIRIN1
Plasmid#107414PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1DepositorInsertKIRIN1
ExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX305_FKBP12F36V-SHOC2
Plasmid#134522PurposeLentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tagDepositorInsertSHOC2 (SHOC2 Human)
UseLentiviralTagsFKBP12F36V-2xHAExpressionMammalianMutationSilent mutations made at wobble base position at …PromoterhPGKAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone
Pooled Library#75312PurposeHuman sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2/LTR7-GFP
Plasmid#62541PurposeLTR7-driving reporterDepositorInsertLTR7-GFP
UseSleeping beauty transposonExpressionMammalianPromoterLTR7Available SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mTurquoise2
Plasmid#191014PurposeGateway entry vector with the mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseGateway entry vectorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CX3CR1-EGFP
Plasmid#24061DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-EGFP
Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBE44
Plasmid#174654PurposeCAPTURE method 15A origin of replication receiver plasmidDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 ALPK1 Y254C
Plasmid#231587PurposeALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged tyrosine 254 to cysteinePromoterdoxycyclineAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRE/VEGF/IRES/EGFP
Plasmid#206211PurposeAn expression vector of VEGF and EGFP under control of TRE promoterDepositorAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV6-Bmal-luc
Plasmid#68833PurposeExpression of Bmal1-luc in mammalian cellsDepositorInsertBmal1 regulatory sequences (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
7xE-Box::Renilla
Plasmid#124532Purposec-Myc Renilla luciferase reporterDepositorInsertc-Myc Renilla reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only