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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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179,538 results

Showing: 9451 - 9500 of 179538 results

  4. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  15. m6A-Tracer-NES

    Plasmid
    #159607
    Purpose
    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.
    Depositor
    Aaron Streets
    Publication
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    m6A-Tracer-NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    159607-plasmid-map-sequence-id-311957

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  18. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    138272-plasmid-map-sequence-id-273617

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  24. pDisplay-mgSrtA

    Plasmid
    #125792
    Purpose
    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
    Depositor
    P. Chen
    Publication
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mgSrtA
    Expression
    Mammalian
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    125792-plasmid-map-sequence-id-258287

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  25. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

    Plasmid
    #125713
    Purpose
    Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet
    Depositor
    Ofer Yizhar
    Publication
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Has service
    AAV1 and AAV5
    Insert
    eOPN3
    Use
    AAV and Cre/Lox
    Tags
    Rho1D4 and mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 331-429
    Promoter
    hSyn1
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    125713-plasmid-map-sequence-id-247207

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  41. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    107024-plasmid-map-sequence-id-208524

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  42. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid
    #100834
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    CamKII
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80 Articles
    100834-plasmid-map-sequence-id-259370

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  44. pAAV-CamKII-ArchT-GFP (PV2527) (AAV1)

    Viral prep
    #99039-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Han et al Front Syst Neurosci. 2011;5:18. Epub 201
    Promoter
    CaMKII
    Tags
    GFP
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  46. pcDNA3.1-hNurr1-NE

    Plasmid
    #102363
    Purpose
    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells
    Depositor
    Shu Leong Ho
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    Tags
    NE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    102363-plasmid-map-sequence-id-200857

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  47. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    103869-plasmid-map-sequence-id-199177

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  50. VN-Tau (P301L)

    Plasmid
    #87634
    Purpose
    expresses hTau fused to the N-terminal part of Venus
    Depositor
    Tiago Outeiro
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPT (MAPT Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Mutation P301L
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87634-plasmid-map-sequence-id-176933

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Showing: 9451 - 9500 of 179538 results