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Showing: 9451 - 9500 of 160219 results

  1. pAD-LyseR

    Plasmid
    #99244
    Purpose
    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    beta-lactamase promoter
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99244-plasmid-map-sequence-id-191038

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  4. pCW57.1 N-term GFP tTA

    Plasmid
    #107551
    Purpose
    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.
    Depositor
    David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP (optional)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107551-plasmid-map-sequence-id-208339

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  5. pDONR223_AKR1B1_WT_V5

    Plasmid
    #82953
    Purpose
    Gateway Donor vector containing AKR1B1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Jesse Boehm, David Root, Matthew Meyerson
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    AKR1B1 (AKR1B1 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82953-plasmid-map-sequence-id-162640

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  7. TFORF0982

    Plasmid
    #143738
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF6 (KLF6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143738-plasmid-map-sequence-id-430116

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  8. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid
    #200410
    Purpose
    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor detecting iRFP720
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200410-plasmid-map-sequence-id-396811

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  9. pGTRm

    Plasmid
    #127197
    Purpose
    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA PCR template
    Use
    Template plasmid for generation of golden gate pc…
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127197-plasmid-map-sequence-id-248423

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  10. pTOX6

    Plasmid
    #127451
    Purpose
    Encodes Tse2 toxin and amilCP chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    tse2 toxin
    amilCP chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    pRha and tac
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127451-plasmid-map-sequence-id-251155

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  11. AAV-CAG-jGCaMP8f-WPRE

    Plasmid
    #179254
    Purpose
    AAV-mediated expression of GCaMP8f under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179254-plasmid-map-sequence-id-381183

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  15. pcDNA3.1-hSyncytin-1(del480)

    Plasmid
    #182441
    Purpose
    hSyncytin-1, deletion 481-538
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deletion 481-538
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182441-plasmid-map-sequence-id-357582

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  16. pAG Retro Puro Empty

    Plasmid
    #234458
    Purpose
    Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.
    Depositor
    Addgene Research Program, Melina Fan
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234458-plasmid-map-sequence-id-464639

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  23. GPR146-DuET

    Plasmid
    #213265
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR146 (GPR146 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213265-plasmid-map-sequence-id-430903

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  25. p663-UBC-miniTurbo-V5-CDK11A_IDG-K

    Plasmid
    #214461
    Purpose
    Gateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK11A (CDK11A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214461-plasmid-map-sequence-id-423211

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  33. Non tagged Avidin

    Plasmid
    #214835
    Purpose
    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Non tagged Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214835-plasmid-map-sequence-id-423116

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  34. TFORF1816

    Plasmid
    #143980
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SATB1 (SATB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143980-plasmid-map-sequence-id-400539

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  35. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-134557

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  36. TFORF2660

    Plasmid
    #142350
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT1 (STAT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142350-plasmid-map-sequence-id-397203

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  37. pAAV-S5E2-dTom-nlsdTom (AAV1)

    Viral Prep
    #135630-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Promoter
    Tags
    dTomato
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  38. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid
    #176750
    Purpose
    AAV-mediated expression of GCaMP8f under the CamKIIa promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176750-plasmid-map-sequence-id-408507

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  42. pTara:500

    Plasmid
    #60717
    Purpose
    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60717-plasmid-map-sequence-id-97574

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  46. FPR2-Tango

    Plasmid
    #66284
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    FPR2 (FPR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66284-plasmid-map-sequence-id-263479

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Showing: 9451 - 9500 of 160219 results