167,825 results

1. pMLS-SV40-EGFP

   Plasmid

   #46919

   Purpose

   Target EGFP gene that is stably integrated into HEK293 genome

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   GFP
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-tTA

   Plasmid

   #104102

   Purpose

   Expresses tTA2 under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pscAAV-CAG-GFP

   Plasmid

   #83279

   Purpose

   recombinant AAV vector packaging self-complementary GFP under the CAG promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   enhanced green fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. YAP/TAZ-TRE-mStrawberry reporter

   Plasmid

   #158682

   Purpose

   This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG vGlut1 mCherry

   Plasmid

   #105010

   Purpose

   Excitatory presynaptic marker

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   SLC17A7
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-EGFP-RhoA-wt

   Plasmid

   #12965

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA (RHOA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

   Plasmid

   #126552

   Purpose

   Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

   Depositor

   Jonathan Karn

   Article

   Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

   Insert

   HIV-1 vector pNL4-3
   

   Use

   Lentiviral

   Tags

   CD8a and GFP

   Mutation

   Delta gag/pol

   Promoter

   HIV LTR

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRAN473

   Plasmid

   #120813

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

   Depositor

   Craig Ellermeier, David S. Weiss

   Article

   Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

   Insert

   red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB405

   Plasmid

   #74799

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSDest

    Plasmid

    #22423

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Type

    Empty backbone

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestattB (pCM268)

    Plasmid

    #68313

    Purpose

    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRZeroT

    Plasmid

    #120276

    Purpose

    Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-DIO-GRAB_OT1.0

    Plasmid

    #185387

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBB HA RelA

    Plasmid

    #74892

    Purpose

    Express RelA in mammalian cells

    Depositor

    Colin Duckett, Ezra Burstein

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    RelA (RELA Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF-1alpha promoter

    Available Since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hUbC-dCas9 KRAB-T2A-GFP

    Plasmid

    #67620

    Purpose

    Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 KRAB T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CAG-jGCaMP8f-WPRE (AAV9)

    Viral Prep

    #179254-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CAG

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

17. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

    Plasmid

    #70231

    Purpose

    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

    Depositor

    Steven Henikoff

    Article

    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

    Insert

    3xFLAG tag and aa 83-231 of Mnase
    

    Use

    Yeast genomic targeting

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cp PS Intein tTA

    Plasmid

    #242026

    Purpose

    Blue-light-controlled release of tTA transactivator from the cytoplasm to the nucleus.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Cp PS Intein tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT040

    Plasmid

    #67640

    Purpose

    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Insert

    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. GFP-TAF1

    Plasmid

    #65395

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TAF1 (TAF1 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJL1

    Plasmid

    #69496

    Purpose

    pJL1 expression vector with sfGFP

    Depositor

    Michael Jewett

    Article

    Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

    Insert

    Super folder GFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pU6-sgGFP-NT1

    Plasmid

    #46914

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgGFP-NT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sfGFP-N1

    Plasmid

    #54737

    Purpose

    Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. TFORF0731

    Plasmid

    #142201

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PROX1 (PROX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-MMLVgag-3xNES-Cas9

    Plasmid

    #181752

    Purpose

    MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLV-Gag-3xNES-Cas9 (Spy)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge