-
Plasmid#127390PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to AlaninePromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar4.3
Plasmid#232743PurposeExpresses NADPH/NADP+ biosensor NAPstar4.3 in S. cerevisiae.DepositorInsertNAPstar4.3
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-mNeongreen
Plasmid#182388PurposeBacterial expression of Vesicle Nucleating peptide6-mNeongreen fusionDepositorInsertVNp6-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB236
Plasmid#68627PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV8)
Viral Prep#50476-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GALT-SEP-mRuby3
Plasmid#200938Purposetrans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pSecUAG_UTu1D
Plasmid#233867PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistantDepositorInsertsallo-tRNA UTu1D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
UseTagsExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG) and …PromoterEM7, araBAD, and native As SelDAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cytoFLARE1.0-TEV
Plasmid#234517PurposeTranscriptional reporter for detecting calcium transients in neuronal cultures (TEVp)DepositorInsertEGFP-P2A-CaM-V5-TEVp
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX208-cytoFLARE1.0-TF
Plasmid#234513PurposeTranscriptional reporter for detecting calcium increase in HEK293T (transcription factor)DepositorInsertERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLAS2w.Phyg-HAVCR1
Plasmid#232669Purposeexpresses HAVCR1 in mammalian cellsDepositorInsertHAVCR1 (HAVCR1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCAG eGFP T2A SP HALO HAPLN1
Plasmid#228200PurposeAAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoterDepositorInsertHAPLN1 (Hapln1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterconstitutive CAG promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (AAV PHP.eB)
Viral Prep#214856-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (#214856). In addition to the viral particles, you will also receive purified AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) plasmid DNA. minBG-driven expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsNoneAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneUseTagsGST (glutathione S-transferase)ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDM069
Plasmid#216821PurposeAspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmStayGold2
UseTagsRIPGLIN and VinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7187
Plasmid#219761PurposeMoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)DepositorInsertPpASCL
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag
Plasmid#216695PurposeSynchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)DepositorInsertsUseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD1-DuET
Plasmid#213227PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD1 (DRD1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-3xCTCF-TetO
Plasmid#213023PurposepiggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposaseDepositorInsert3xCTCF-TetO
UseMouse Targeting; Piggybase integration (transposo…TagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-RIOK1_IDG-K
Plasmid#214464PurposeGateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-RIOK1 (RIOK1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_LEF1_TAF8
Plasmid#205846PurposeExpress mEGFP-tagged fusion protein, LEF1_TAF8 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 LARP7-FLAG 7SK
Plasmid#200733PurposeLentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SKDepositorInsertLARP7 and 7SK (LARP7 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterU6Available sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET41c-PhTET2
Plasmid#182428PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coliDepositorInsertTetrahedral aminopeptidase TET2 from P. hirokoshii
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
DCAF1:PBC020-D05:C246337
Plasmid#183974PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertDCAF1:PBC020-D05:C246337 (DCAF1 Human)
UseBaculovirus expresssionTags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionMutationPromoterPolyhedrinAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)
Plasmid#181760Purposetargeting vectorDepositorInsertnls-tagged EGFP and nls-tagged dTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-XTbx5-EnR
Plasmid#117249PurposemRNA synthesisDepositorInsertTbx5-EnR (tbx5-b Frog)
UseXenopus expressionTagsExpressionMutationchimeric protein with engrailed repressor domain…PromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-RA
Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddRFP A
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE
Plasmid#162379PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR_LG lenti-(empty)-del-CTS
Plasmid#112895PurposeNon-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid librariesDepositorArticleTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoternoneAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs
Plasmid#106908Purposefull-length murine SSeCKs/AKAP12/Gravin in retroviral vectorDepositorInsertSSeCKs (Akap12 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromotergagAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
tph-1(short)::rpl-22-HA
Plasmid#122203PurposeC. elegans tagged ribosome expression plasmidDepositorInserttph-1(short)::rpl-22-HA
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
S96 ELP
Plasmid#68393PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residueDepositorInsertS96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-EGFP (AAV8)
Viral Prep#50469-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEGFPAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only