We narrowed to 18,838 results for: RAN-1

1. pLNHA-C1-Hs4E-BP1

   Plasmid

   #79438

   Purpose

   Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Hs4E-BP1 (EIF4EBP1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-F-AF9

   Plasmid

   #49428

   Purpose

   Mammalian expression of Flag-tagged human AF9

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   AF-9 (MLLT3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX SH3(2)-3R

   Plasmid

   #112091

   Purpose

   Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-3R (NCK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9-HE-Geminin

   Plasmid

   #109402

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HE-Geminin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21a Ov-iPGM

   Plasmid

   #180245

   Purpose

   Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   Onchocerca volvulus iPGM-10His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a Bm-iPGM-bio

   Plasmid

   #162565

   Purpose

   Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   B. malayi iPGM bio-6His
   

   Use

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF420

   Plasmid

   #123659

   Purpose

   Produces cofactor F420 in E. coli

   Depositor

   Colin Jackson

   Article

   Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

   Inserts

   CofD
   FbiB
   FbiD
   FbiC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_R0040

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB CAG Otx2-IRES-EGFP

   Plasmid

   #153947

   Purpose

   piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Otx2 (Otx2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-Flag-Narg2

    Plasmid

    #49247

    Purpose

    mammalian expression of flag-tagged human Narg2

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Narg2 (NARG2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)-human LRRC33

    Plasmid

    #111602

    Purpose

    Express human LRRC33 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    human LRRC33 (NRROS Human)
    

    Use

    Tags

    myc/his tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EID1-pGex-2TK

    Plasmid

    #22689

    Depositor

    William Kaelin

    Article

    Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

    Insert

    E1A-like inhibitor of differentiation 1 (EID1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

    Plasmid

    #199457

    Purpose

    Expression of dCas13-VPR in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    dCas13
    

    Use

    Lentiviral

    Tags

    VPR-T2A-HaloTag

    Expression

    Mammalian

    Mutation

    Nuclease-dead H133A and H1058A

    Promoter

    CAG

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG668 lifeAct-pFAST

    Plasmid

    #172866

    Purpose

    Expresses LifeAct-pFAST in mammalian cells (actin labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    LifeAct-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.CMV.RGS4.C-HA

    Plasmid

    #154847

    Purpose

    Expresses murine RGS4 protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Regulator of G protein Signaling 4 (Rgs4 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5HT6-YFP-ThymosinBeta4(KK18,19EE)

    Plasmid

    #96807

    Purpose

    non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    KK18,19EE prevents G-actin binding

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CaV1.3e[8a,11,31b,Δ32,42a] mut

    Plasmid

    #26576

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 TREK-2

    Plasmid

    #224769

    Purpose

    In vitro transcription for Oocyte expression of 1 TREK-2

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 10 (Kcnk10 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmIL-6 mut C/EBP

    Plasmid

    #61291

    Purpose

    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated C/EBP binding site from ACATTGTGCAATCT to…

    Promoter

    IL-6 promoter with mutant C/EBP binding site

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAAV.CMV.VTN.C-HA

    Plasmid

    #154849

    Purpose

    Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Vitronectin (Vtn Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-IP3KA

    Plasmid

    #32529

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KA (ITPKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pAG671 mito-pFAST

    Plasmid

    #172867

    Purpose

    Expresses mitochondrial targeting domain-pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    mito-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG657 H2B-pFAST

    Plasmid

    #172862

    Purpose

    Expresses pFAST fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    H2B-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDRf1-4CL5-AtSCT (JBEI-11557)

    Plasmid

    #87937

    Purpose

    Co-expression in S.cerevisiae of 4CL5 and AtSCT

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Inserts

    AtSCT
    4CL5 (4CL5 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG665 MAP4-pFAST

    Plasmid

    #172865

    Purpose

    Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    MAP4-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GST-PS-Sos1 (PRM) - Cys (XSB364)

    Plasmid

    #78506

    Purpose

    Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Sos1 -Cys (PRM) (SOS1 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MDH1-miR-181a1-Dusp6

    Plasmid

    #17849

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    Dusp6-EGFP (Dusp6 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ET1-human LRRC33

    Plasmid

    #111603

    Purpose

    Express human LRRC33 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    human LRRC33 (NRROS Human)
    

    Use

    Tags

    PreScission/TEV/ProteinC/6xHIS/SBP/

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Jag2ICD-HA pBacPAK8-3

    Plasmid

    #17332

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged2 (Jag2 Rat)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

30. MDH1-shDusp5-2-H2Kb2

    Plasmid

    #17853

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shDusp5 (DUSP5 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Jag1ICD-HA pBacPAK8-3

    Plasmid

    #17331

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged1 (Jag1 Rat)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

32. 33-G-X1-pEF1-puro

    Plasmid

    #111604

    Purpose

    Express human 33-G-X1 in 293T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    human LRRC33 and GARP chimeria (NRROS Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Djun - sense

    Plasmid

    #38337

    Depositor

    Norbert Perrimon

    Article

    Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

    Insert

    djun (Jra Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

34. Dll3ICD-HPC4 pBacPAK8-3

    Plasmid

    #17334

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Delta-like 3 (Dll3 Mouse)
    

    Use

    Tags

    HPC4

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

35. Djun - antisense

    Plasmid

    #38338

    Depositor

    Norbert Perrimon

    Article

    Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

    Insert

    djun (Jra Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

36. Neurl1-FLAG/Jag1-HA pIRES

    Plasmid

    #17337

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Neuralized-like (Neurl1a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

37. Neurl1G2A-Flag pcDNA3.1

    Plasmid

    #17320

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Neuralized-like (Neurl1a Mouse)
    

    Use

    RNAi

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Gly 2 changed to Ala

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

38. pCMV-MMLVgag-D3A-3L-dCas9-KOX1

    Plasmid

    #240532

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-KOX1
    

    Use

    Tags

    FLAG, HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFUW-TetO-MCS

    Plasmid

    #210527

    Purpose

    doxycycline-inducible expression of Human inserts (MCS) in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHAGE2-TetO-Chim-Salhel-I-K57E

    Plasmid

    #231519

    Purpose

    Expresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Salhel-I HMG K57E flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation at position 57 K to E of the HMG

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET21a Di-iPGM

    Plasmid

    #180241

    Purpose

    Bacterial expression plasmid for production of recombinant Dirofilaria immitis iPGM His10

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    Dirofilaria immitis iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA-FLAG-Co-RHD

    Plasmid

    #180198

    Purpose

    Expresses FLAG-tagged version of the Co-NF-kB Rel Homology Domain

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-RHD
    

    Use

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-582

    Promoter

    CMV

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRAR039

    Plasmid

    #185025

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 1-31 deleted from rpmE 5'UTR, On…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pHD22Y/PfPRMT5 deletion

    Plasmid

    #203161

    Purpose

    for disruption of PfPRMT5 gene

    Depositor

    Liwang Cui

    Article

    Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

    Insert

    PfPRMT5 fragment
    

    Use

    Tags

    3 myc tags and 3' UTR of P. berghei dihydrof…

    Expression

    Mammalian

    Mutation

    Promoter

    No

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. FUW-MDmut-mTurquoise

    Plasmid

    #193095

    Purpose

    constitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    MDmut-mTurquoise
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA-FLAG-Co-NF-kB

    Plasmid

    #180197

    Purpose

    Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Use

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-1224

    Promoter

    CMV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET21a Ec-iPGM-bio

    Plasmid

    #162575

    Purpose

    Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    E. coli iPGM bio-6His
    

    Use

    Tags

    6His tag, T7 tag, biotinylation sequence, and thr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pQE30-HypocratesCS

    Plasmid

    #161957

    Purpose

    Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

    Depositor

    Dmitry Bilan

    Article

    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

    Insert

    HypocratesCS
    

    Use

    Tags

    6xHIs tag

    Expression

    Bacterial

    Mutation

    changed cysteine 355 to serine

    Promoter

    T5

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pETDUET-GST AO7RE Y165A

    Plasmid

    #139428

    Purpose

    GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed tyrosine 165 to an alanine

    Promoter

    T7 promoter-1

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pETDUET-GST AO7RE P199A

    Plasmid

    #139427

    Purpose

    GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed proline 199 to an alanine

    Promoter

    T7 promoter-1

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge