We narrowed to 15,976 results for: LEA

1. pTP396

   Plasmid

   #149336

   Purpose

   Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

   Depositor

   Rebecca Voorhees

   Article

   Pleiner et al Science. 2020 May 21. pii: science.abb50

   Insert

   anti-GFP nanobody
   

   Tags

   Biotin acceptor peptide (Avi-tag), His14, and SUM…

   Expression

   Bacterial

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAR-Ec318

   Plasmid

   #130871

   Purpose

   A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

   Depositor

   Chang Liu

   Article

   Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

   Insert

   TP-DNAP1
   

   Expression

   Yeast

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTP1122

   Plasmid

   #104159

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MBP-TEV

   Plasmid

   #69929

   Purpose

   bacterial expression of MBP-TEV n-terminal fusions

   Depositor

   Thomas Wassmer, Zita Balklava

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   MBP and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZipGFP-Casp3

   Plasmid

   #81241

   Purpose

   A ZipGFP-based Caspase-3 protease reporter T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

   Insert

   ZipGFP with caspase cleavage sequence T2A mCherry
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTP1183

   Plasmid

   #104163

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-CaNAR2

   Plasmid

   #64728

   Purpose

   FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Tags

   Cerulean3 and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDN3.1(+)-CaNARi

   Plasmid

   #64848

   Purpose

   FRET-based calcineurin (CaN) activation biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. nls-mCherry

    Plasmid

    #108881

    Purpose

    Expression of nuclear localized mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    nls-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTP955

    Plasmid

    #104164

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid

    #228469

    Purpose

    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Inserts

    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    

    Expression

    Bacterial

    Promoter

    J23106, J23108, and T7A1

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRel

    Plasmid

    #227581

    Purpose

    Release plasmid with Cas9 working cassette, apramycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-HT-emiRFP703

    Plasmid

    #183985

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Tags

    HaloTag, emiRFP703

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPROEX Aqua

    Plasmid

    #42889

    Depositor

    Fabienne Merola

    Article

    Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE2_CMV_Mex3a_t2a_mCherry

    Plasmid

    #223244

    Purpose

    tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry

    Depositor

    Stavros Lomvardas

    Insert

    Mex3a (Mex3a Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    amino acids conserved, underlying DNA sequence re…

    Promoter

    CMV

    Available Since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTP1112

    Plasmid

    #104158

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST14-SpyCatcher002

    Plasmid

    #102827

    Purpose

    Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST14-DogCatcher

    Plasmid

    #171772

    Purpose

    Expresses DogCatcher protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTH2

    Plasmid

    #84452

    Purpose

    shuttle plasmid for sarA P1-CFP expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    CFP Cerulean
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-Tre3G-29xGGGGCC-12xMS2

    Plasmid

    #99149

    Purpose

    Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    29xGGGGCC 12xMS2 hairpins
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-HIFR

    Plasmid

    #192946

    Purpose

    Lentiviral production of HIF-1alpha reporter

    Depositor

    Markus Covert

    Article

    Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

    Insert

    HIF-1alpha reporter
    

    Use

    Lentiviral

    Promoter

    mCMV

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PKCalpha.FLAG

    Plasmid

    #10805

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D332G-please see comments below

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNCS YFP

    Plasmid

    #91759

    Purpose

    Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

    Insert

    Venus YFP (A206K)
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-AktAR2

    Plasmid

    #64932

    Purpose

    Enhanced FRET-based biosensor for monitoring Akt activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    AktAR2
    

    Tags

    Cerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJ404-DogCatcher-sfGFP

    Plasmid

    #171930

    Purpose

    Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher-sfGFP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-OSF-PP

    Plasmid

    #154247

    Purpose

    backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Type

    Empty backbone

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. UAS-Syt::7xHalo7

    Plasmid

    #87650

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::7xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::7xHalo7
    

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-iGlu3Fast

    Plasmid

    #226500

    Purpose

    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 S72T
    

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    S72T

    Promoter

    CMV

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

    Plasmid

    #175544

    Purpose

    Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag9-myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MLM3613

    Plasmid

    #42251

    Purpose

    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJExpress414_GmPOPB-S577A

    Plasmid

    #92235

    Purpose

    Express S577A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-S577A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Serine 577 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJExpress414_GmPOPB-D661A

    Plasmid

    #92236

    Purpose

    Express D661A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-D661A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Aspartate 661 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHB-4

    Plasmid

    #140957

    Purpose

    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJExpress414_GmPOPB-H698N

    Plasmid

    #92242

    Purpose

    Express H698N mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-H698N mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Histidine 698 to Asparagine

    Promoter

    T7 promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTP1005

    Plasmid

    #104160

    Purpose

    Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pKLBac-Bat-FluA-trimer

    Plasmid

    #229858

    Purpose

    Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

    Inserts

    PA_Bat-FluA_AFC35437.1
    PB1_Bat-FluA_AFC35436
    PB2_Bat-FluA_AFC35435.1
    

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBbAW4k-loxP-TT-loxP-mRFP1

    Plasmid

    #134405

    Purpose

    Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    W4-loxP-TT-loxP-mRFP1
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Bacterial

    Promoter

    W4 (modified T7A1)

    Available Since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. bZiPro

    Plasmid

    #86846

    Purpose

    express NS2B-NS3 protease in E.coli

    Depositor

    Dahai Luo

    Article

    Zhang et al Science. 2016 Dec 8. pii: aai9309.

    Inserts

    NS3 1-177
    NS2B45-96
    

    Tags

    a hexahistidine tag and a thrombin cleavage site

    Expression

    Bacterial

    Mutation

    NS2B cofactor region (residues 45–96) and NS3 pro…

    Promoter

    T7

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRK792

    Plasmid

    #8830

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219P mutant
    

    Tags

    His, MBP (cleaved), and polyarginine

    Expression

    Bacterial

    Mutation

    S219P mutation

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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50. pQE30-His-RF3

    Plasmid

    #124131

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 3 (prfC Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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