163,331 results

1. pT3-EF1aH c-Met

   Plasmid

   #86498

   Purpose

   Mammalian expression of human c-Met

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-FOLD6

   Plasmid

   #186547

   Purpose

   FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   FOLD6
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

   Promoter

   araB

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV pmSyn1-EBFP-Cre

   Plasmid

   #51507

   Purpose

   Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV5

   Insert

   EBFP-Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pmSyn1

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EF1aL-eGFP-W

   Plasmid

   #126686

   Purpose

   Constitutive, ubiquitous GFP expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENN.AAV.CAG.tdTomato.WPRE.SV40

   Plasmid

   #105554

   Purpose

   AAV expression of tdTomato from CAG promoter

   Depositor

   James M. Wilson

   Insert

   tdtomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREVtre GFP

   Plasmid

   #128069

   Purpose

   Retroviral tet inducible EGFP

   Depositor

   Oskar Laur

   Insert

   tet inducible EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   no

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CXCR7-Tango

   Plasmid

   #66265

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CXCR7 (ACKR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-G-Creon shRNA[Control]

    Plasmid

    #181824

    Purpose

    This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Promoterless

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFlag-Rab34

    Plasmid

    #232458

    Purpose

    Expresses 3xFlag-tagged human Rab34 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    Rab34 (RAB34 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDE43-MCSq17

    Plasmid

    #27333

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    qtag17
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. hBax C3-EGFP

    Plasmid

    #19741

    Depositor

    Richard Youle

    Article

    Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

    Insert

    Bax (BAX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCVII-mChe

    Plasmid

    #162753

    Purpose

    Retroviral (MSCV) expression of mCherry fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCVII-E2Crimson

    Plasmid

    #162756

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)

    Viral Prep

    #162380-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Tags

    None

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

19. ERmoxGFP

    Plasmid

    #68072

    Purpose

    mammalian expression of ER localized moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    moxGFP
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV GFP DEST (736-1)

    Plasmid

    #19732

    Purpose

    3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA5-FRT/TO-eGFP-53BP1

    Plasmid

    #60813

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pcDNA3.1-AXL-V5/HIS

    Plasmid

    #200117

    Purpose

    Expression of human AXL receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human AXL receptor tyrosine kinase (AXL Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBMN FKBP(DD)-YFP

    Plasmid

    #31763

    Depositor

    Thomas Wandless

    Article

    Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

    Insert

    FKBP(DD)-YFP-HA (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    F36V, L106P on FKBP12

    Promoter

    LTR

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCDNA3-HA-Akt1

    Plasmid

    #73408

    Purpose

    Expresses human AKT1 with a N-terminus HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131004

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

    Plasmid

    #85431

    Purpose

    Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human Tbet
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSP3535

    Plasmid

    #46886

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Farnselation tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCVII-AM

    Plasmid

    #162751

    Purpose

    Retroviral (MSCV) expression of ametrine fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    ametrine
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LeGO-E

    Plasmid

    #27359

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, EmeraldGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge