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Showing: 10901 - 10950 of 160219 results

  1. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209416-plasmid-map-sequence-id-416613

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  5. MLRV

    Plasmid
    #118069
    Purpose
    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Inserts
    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    118069-plasmid-map-sequence-id-240503

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  8. pAAV_BiSSTe10_ChR2_mCherry (AAV PHP.eB)

    Viral Prep
    #213815-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiSSTe10_ChR2_mCherry (#213815). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the SST interneuron-targeting enhancer E10. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Promoter
    Tags
    mCherry
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV.GFAP.iGluSnFR3.v857.GPI

    Plasmid
    #178338
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV GFAP iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    178338-plasmid-map-sequence-id-361534

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  14. AAV-mDlx-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #176753-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-mDlx-jGCaMP8f-WPRE (#176753). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8f-WPRE plasmid DNA. mDlx-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    mDlx
    Tags
    None
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  20. mRuby-Cx32-7

    Plasmid
    #55855
    Purpose
    Localization: Gap Junctions, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Cx32 (GJB1 Human)
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55855-plasmid-map-sequence-id-175697

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  22. pLIX-hN1ICD

    Plasmid
    #91897
    Purpose
    Doxycycline inducible expression of human Notch1 ICD
    Depositor
    Julien Sage
    Insert
    Notch1 intracellular domain (NOTCH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codons 1770 to 2555 of human NOTCH1
    Promoter
    TRE promoter, Tet ON
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91897-plasmid-map-sequence-id-180657

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  23. pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV1)

    Viral Prep
    #100841-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. TFORF3448

    Plasmid
    #144924
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144924-plasmid-map-sequence-id-404484

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  27. pAAV_hSyn1-SIO-stGtACR1-FusionRed (AAV5)

    Viral Prep
    #105678-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn1-SIO-stGtACR1-FusionRed (#105678). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR1-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only

  46. pRRlsinPGK_H2BGFP_WPRE

    Plasmid
    #91788
    Purpose
    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    Human histone H2B (H2BC11 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91788-plasmid-map-sequence-id-180614

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  49. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid
    #229997
    Purpose
    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229997-plasmid-map-sequence-id-457203

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Showing: 10901 - 10950 of 160219 results