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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,976 results for: LEA

Showing: 10901 - 10950 of 15976 results

  10. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

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  26. 2xMARS

    Plasmid
    #205233
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205233-plasmid-map-sequence-id-404744

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  27. pAPV-DsRed2-ER-5

    Plasmid
    #170570
    Purpose
    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170570-plasmid-map-sequence-id-335779

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  33. FnR8-10

    Plasmid
    #182569
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182569-plasmid-map-sequence-id-357183

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  36. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  42. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

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  46. pcDNA3-StagRFP

    Plasmid
    #173014
    Purpose
    Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Insert
    StagRFP
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173014-plasmid-map-sequence-id-343313

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  47. HECTD1

    Plasmid
    #32860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECTD1 (HECTD1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 1879-1966 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. BRD7 (BRD7A-c005)

    Plasmid
    #98245
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD7A (BRD7 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00439:Bromodomain 136-223
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98245-plasmid-map-sequence-id-193423

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Showing: 10901 - 10950 of 15976 results