We narrowed to 18,161 results for: Met;

1. pACYCDuet_F31YG121VDHFR_WTTS

   Plasmid

   #91229

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F31YG121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACYCDuet_G121VDHFR_WTTS

   Plasmid

   #91233

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPD385 VPR-ZF43(RAAA)

   Plasmid

   #138824

   Purpose

   Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-ZF43(RAAA)
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   R15A, R43A, R71A

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. JEH127

   Plasmid

   #179299

   Purpose

   Expresses dCas9 fused to VPR

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a - Mtb-TF

   Plasmid

   #111513

   Purpose

   Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   Trigger Factor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos2-Calnexin-N-14

   Plasmid

   #57352

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pVH232-Tier1-PmPGK1-mRuby2

   Plasmid

   #169523

   Purpose

   Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric red fluorescent protein mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGas-miRFP670nano3

   Plasmid

   #184672

   Purpose

   G protein alpha-subunit internally tagged with miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   G protein alpha-s-miRFP670nano3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos2-ER-5

   Plasmid

   #57373

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   ER (CALR Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIR-D10 UTR

    Plasmid

    #19117

    Depositor

    Bob Weinberg

    Article

    Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

    Insert

    HOXD10 3' UTR (HOXD10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

12. MXS_MCS1

    Plasmid

    #62395

    Purpose

    MXS_chaining vector with MCS1

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. MXS_CMV::tTA2-bGHpA

    Plasmid

    #62447

    Purpose

    MXS_chaining vector with CMV::tTA2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tetracycline transactivator with CMV promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM6-At4CL-PhCHS-CmCHI

    Plasmid

    #73393

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. C413-E22: attB4-polyhedrinp>-attB1r

    Plasmid

    #162922

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPT-PCR C2

    Plasmid

    #141247

    Purpose

    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Algal Research, 2020. 50, 102014

    Insert

    Mitochondrial Genome of Phaeodactylum tricornutum
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Repeat region removed, additional minor mutations…

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUCBB-pBAD-eGFP

    Plasmid

    #32553

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFU-cMVIIA-PE

    Plasmid

    #24147

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Feb 7. ():.

    Insert

    loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD+Red-F

    Plasmid

    #170874

    Purpose

    Phytobrick - Red-F Luciferase CDS

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    p.S286Y Red mutant

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid

    #168207

    Purpose

    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Type

    Empty backbone

    Use

    Unspecified; Pdest gateway

    Tags

    V5 APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXWJ109As-gfp

    Plasmid

    #123143

    Purpose

    An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Dendra2-EB3-7

    Plasmid

    #57715

    Purpose

    Localization: MT End Binding Protein, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pSB1K3_pBen-sfGFP_J23101-BenR

    Plasmid

    #128125

    Purpose

    Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    sfGFP and BenR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBen and J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mEos2-Vimentin-7

    Plasmid

    #57435

    Purpose

    Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pCDNA3.0_mitoNanobody IRES EGFP

    Plasmid

    #136621

    Purpose

    Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA5/FRT-ChemoB-NAD

    Plasmid

    #193824

    Purpose

    CMV overexpression of ChemoB-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. C455-E01: attB4-stuffer-attB5

    Plasmid

    #162983

    Purpose

    Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. C515-E01: attB5r-stuffer-attB1r

    Plasmid

    #162968

    Purpose

    Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MXS_PGK::NeoR-bGHpA

    Plasmid

    #62438

    Purpose

    MXS_chaining vector with PGK::NeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Neomycin with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-smURFP-P2A-GFP

    Plasmid

    #197151

    Purpose

    Expresses the protein of smURFP-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    smURFP-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mEos2-CathespinB-6

    Plasmid

    #57354

    Purpose

    Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    CathespinB (Ctsb Rat)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pLBC0010

    Plasmid

    #172774

    Purpose

    Level 0 module containing NT1_CTPSSU

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLBC0011

    Plasmid

    #172775

    Purpose

    Level 0 module containing NT2_CTPSSU

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLBC0060

    Plasmid

    #172783

    Purpose

    Level 0 module containing NT2_cYFP3XHA

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS C-terminal eYFP (175-238) 3XHA tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLBC0061

    Plasmid

    #172784

    Purpose

    Level 0 module containing CT_cYFP3XHA

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS C-terminal eYFP (175-238) 3XHA tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)

    Plasmid

    #104117

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-dark Citrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti_CMV:Flag-dKeNLuc_SV40:PuroR

    Plasmid

    #183046

    Purpose

    Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    dKeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti_CMV:Flag-KaNLuc_SV40:PuroR

    Plasmid

    #183047

    Purpose

    Expresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    KaNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSuper.Retro.Puro-WDR5 shRNA 2

    Plasmid

    #59975

    Purpose

    Knockdown of WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 shRNA
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pETM6-Vv4CL-PhCHS-CmCHI

    Plasmid

    #73401

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge