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We narrowed to 47,600 results for: CHI

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Showing: 11001 - 11050 of 47600 results

  4. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  6. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100285-plasmid-map-sequence-id-194439

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  8. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available Since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  9. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  15. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  22. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid
    #202531
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    DGKZ (DGKZ Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202531-plasmid-map-sequence-id-399624

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  23. pCAGGS-PHFF-sh-mBVRA

    Plasmid
    #100286
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available Since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100286-plasmid-map-sequence-id-194443

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  33. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

    Plasmid
    #72608
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine37 to alanine
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  43. pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

    Plasmid
    #72609
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed threonine34 to alanine
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  44. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

    Plasmid
    #72610
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33 to alanine
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid
    #72613
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Changed serine37 to alanine
    Promoter
    TRE tight promoter
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72613-plasmid-map-sequence-id-138777

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  46. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid
    #72611
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33, threonine34 and serine37 to ala…
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 11001 - 11050 of 47600 results