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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,271 results

Showing: 8881 - 8920 of 161271 results

  1. TFORF0361

    Plasmid
    #142255
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCEB3 (ELOA Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142255-plasmid-map-sequence-id-395748

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  4. pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9)

    Viral Prep
    #112007-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (#112007). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6s and bicistronic mRuby3. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Tags
    mRuby3
    Available since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  8. pR26 CAG/GFP Asc

    Plasmid
    #74285
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74285-plasmid-map-sequence-id-357226

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  9. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)

    Viral Prep
    #105677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  11. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  13. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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  15. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134908-plasmid-map-sequence-id-342556

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  23. pTrEno

    Plasmid
    #115474
    Purpose
    Eno-promoter driven expression vector for protein production in Trichoderma reesei
    Depositor
    Steve Decker
    Article
    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d
    Insert
    Cel7A
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Introns have been removed
    Promoter
    Trichoderma reesei Enolase (Eno) promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    115474-plasmid-map-sequence-id-225594

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  28. pLenti6.2-Nanoluc-ccdB

    Plasmid
    #87078
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87078-plasmid-map-sequence-id-173606

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  31. Tet-pLKO-FOXM1-shRNA-81

    Plasmid
    #89497
    Purpose
    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    June 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    89497-plasmid-map-sequence-id-181253

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Showing: 8881 - 8920 of 161271 results