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171,853 results

Showing: 11301 - 11350 of 171853 results

  1. pMSP12::mCherry

    Plasmid
    #30169
    Depositor
    Lalita Ramakrishnan
    Insert
    Mycobacterium Strong Promoter (MSP)
    Use
    Mycobacteria expression
    Tags
    mCherry
    Available Since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    30169-plasmid-map-sequence-id-214699

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  10. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (AAV1)

    Viral Prep
    #98928-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (#98928). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of Ruby2sm-Flag spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:
    Promoter
    CAG
    Tags
    mRuby2sm-Flag (spaghetti monster construct)
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

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  13. TFORF0885

    Plasmid
    #142210
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARG (PPARG Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142210-plasmid-map-sequence-id-397707

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  14. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

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  18. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  20. pAAV-CAG-H2B tdTomato (AAV Retrograde)

    Viral Prep
    #116870-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-H2B tdTomato (#116870). In addition to the viral particles, you will also receive purified pAAV-CAG-H2B tdTomato plasmid DNA. CAG-driven expression of H2B-tdTomato. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1
    Tags
    tdTomato
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  21. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Retroviral 5' LTR promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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  29. pDsRed-attP

    Plasmid
    #51019
    Purpose
    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Article
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    Use
    High copy, amp resistance
    Promoter
    3xP3 and None
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51019-plasmid-map-sequence-id-222558

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  30. GPR37-DuET

    Plasmid
    #213282
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR37 (GPR37 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213282-plasmid-map-sequence-id-430904

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  37. LentiV_Neo_SIK3_CR_K95M

    Plasmid
    #108106
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108106-plasmid-map-sequence-id-207840

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  41. TFORF1106

    Plasmid
    #143762
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EPAS1 (EPAS1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143762-plasmid-map-sequence-id-385793

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Showing: 11301 - 11350 of 171853 results