161,697 results
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SPLICS NU-MT P2A
Plasmid#210129PurposeDetect the Nucleus-Mitochondria contactDepositorInsertSPLICS NU-MT P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-KRasCT
Plasmid#175835PurposeExpress mCherry with plasma membrane-targeting sequence in mammalian cellsDepositorInsertmCherry with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_EML4_ALK
Plasmid#205809PurposeExpress mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW103ParsR-Amp30C
Plasmid#78638PurposepBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsert30hrpR-30hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAd-M3cherry
Plasmid#61041PurposeThis plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.DepositorUseAdenoviralTagsExpressionMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrEno
Plasmid#115474PurposeEno-promoter driven expression vector for protein production in Trichoderma reeseiDepositorInsertCel7A
UseUnspecifiedTagsExpressionMutationIntrons have been removedPromoterTrichoderma reesei Enolase (Eno) promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-HsNRF2 (NFE2L2)
Plasmid#194303PurposeMammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTHDepositorInsertNFE2L2 (NFE2L2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAA)20-P2A-RFP
Plasmid#105688PurposeDouble fluorescent stalling reporter K(AAA)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV CD68-hM4D(Gi)-mCherry
Plasmid#75033PurposeExpression of hM4D(Gi) using the CD68 promoter.DepositorHas ServiceAAV1 and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsExpressionMammalianMutationPromoterCD68Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)
Plasmid#224052PurposeAiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neuronsInsertmScarlet
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-wtATMSPARK2
Plasmid#195247Purposeexpress wtATMSPARK2DepositorInsertATMSPARK2 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK GFP Puro (w509-5)
Plasmid#19070Purpose3rd gen lentiviral eGFP expression vector, PGK promoter, PuroDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flamindo2
Plasmid#73938PurposeExpresses biosensor for cAMP in mammalian cellsDepositorInsertFlamindo2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-C
Plasmid#118369PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-GFP-CIBN
Plasmid#102252PurposeExpresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP4
Plasmid#42313DepositorInsertVAMP 4 (VAMP4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-pBAD
Plasmid#54856PurposeLocalization: Protein Expression Vector , Excitation: 554, Emission: 581DepositorTypeEmpty backboneUseTags6xHis and tdTomatoExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
GRM5-DuET
Plasmid#213303PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM5 (GRM5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-RatLC3B-C-7
Plasmid#55128PurposeLocalization: Microtubule-Binding Protein, Excitation: 587, Emission: 610DepositorInsertLC3B
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-P4Mx1
Plasmid#108143PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1 D256
Plasmid#12145DepositorInsertFoxo1 (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationContains a deletion truncating the protein after …PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Integrin-Alpha2-N-18
Plasmid#55063DepositorInsertIntegrin-Alpha2 (ITGA2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUV15tetORm
Plasmid#17975DepositorInsertpmyc1tetO-gfp, tetR(B)
UseMycobacteria expressionTagsExpressionMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
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p-cyclinB1-mcherry (1958)
Plasmid#26063DepositorInsertCyclin B1 (CCNB1 Human)
UseTagsmcherryExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
Pol
Plasmid#65520PurposeExpresses HBV polymerase in mammalian cellsDepositorInsertHBV polymerase protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
Plasmid#196196Purposehomologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
UseHomology repair donorTagsV5-APEX2-mScarlet-IExpressionMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Cdc42-C1
Plasmid#29673DepositorInsertCdc42 (CDC42 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR' CMV GFP
Plasmid#14858DepositorInsertCMV-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
p6344 pcDNA4-TO-HA-Brd4FL
Plasmid#31351DepositorInsertBrd4FL
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_hsTRPA1_myc_dTom
Plasmid#183179Purposemyc tagged hsTRPA1 used for cell transfectionDepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcB
Plasmid#51282PurposeE. coli expression vector for E. coli RtcB RNA ligaseDepositorInsertE. coli RtcB
UseTags6xHisExpressionBacterialMutationPromoterT7 RNA PolymeraseAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p6sheLLr
Plasmid#37318DepositorInsertHPV6L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Zeo
Plasmid#227258PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS648∙CsR
Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2 kb prom. CD274
Plasmid#107003PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBsacA
Plasmid#158650PurposeConstitutive transcription of sacA crRNADepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterPvegAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL21-mCherry
Plasmid#164410PurposeRetroviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus2ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
