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pcDNA3/Myc-DNMT1

Plasmid

#36939

Purpose

Mammalian expression of human DNMT1 with myc tag

Depositor

Arthur Riggs

Publication

Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

Insert

DNMT1 (DNMT1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pCMVHA hEZH2

Plasmid

#24230

Purpose

Mammalian expression of human EZH2 with HA tag

Depositor

Kristian Helin

Publication

Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

Insert

EZH2 (EZH2 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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pDEST-HisMBP

Plasmid

#11085

Depositor

David Waugh

Publication

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis and MBP

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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mCh-Sec61 beta

Plasmid

#49155

Purpose

expression of Sec61 beta fused to mCherry, used as a general ER marker

Depositor

Gia Voeltz

Publication

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Tags

mCherry*

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

64 Articles

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PGC-1 alpha promoter 2kb luciferase

Plasmid

#8887

Depositor

Bruce Spiegelman

Publication

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pcDNA3-EGFP-RhoA-T19N

Plasmid

#12967

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA DN (RHOA Human)

Tags

EGFP

Expression

Mammalian

Mutation

T19N Dominant negative

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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mKeima-Red-Mito-7

Plasmid

#56018

Purpose

Localization: Mitochondria, Excitation: 440, Emission: 620

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

mKeima-Red

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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MoClo Tool Kit

Plasmid kit

#1000000044

Purpose

Collection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs.

Depositor

Sylvestre Marillonnet

Application

Cloning and Synthetic Biology

Vector type

Eukaryotic Expression

Cloning type

Golden Gate (MoClo)

Available since

April 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pMS2-GFP

Plasmid

#27121

Depositor

Robert Singer

Publication

Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

Insert

MS2

Tags

EGFP, HA, and SV40 NLS

Expression

Mammalian

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pBABEpuro HA Brca1

Plasmid

#14999

Depositor

Stephen Elledge

Publication

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

Brca1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pMSCV-IRES-mCherry FP

Plasmid

#52114

Purpose

a bicistronic IRES-mCherry containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pCAG-T7pol

Plasmid

#59926

Purpose

Expression vector for T7 RNA polymerase gene

Depositor

Ian Wickersham

Insert

T7 RNA polymerase

Expression

Mammalian

Promoter

CAG

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pLAMP1-mCherry

Plasmid

#45147

Depositor

Amy Palmer

Publication

Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

Insert

LAMP1-mCherry (LAMP1 Human, Synthetic)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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nls-NgAgo-GK

Plasmid

#78253

Purpose

Mammalian expression of NgAgo (Argonaute from N. gregoryi).

Depositor

Chunyu Han

Publication

Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

NgAgo

Tags

a nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pEGFP-C1-ER alpha

Plasmid

#28230

Purpose

Mammalian expression of Estrogen Receptor alpha fused to EGFP

Depositor

Michael Mancini

Publication

Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

Insert

Estrogen Receptor alpha (ESR1 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Tags

Myc

Expression

Mammalian

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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HDAC3 Flag

Plasmid

#13819

Purpose

Mammalian expression of human histone deacetylase 3 with flag tag

Depositor

Eric Verdin

Publication

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC3 (HDAC3 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pTet-O-Ngn2-puro

Plasmid

#52047

Purpose

3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Publication

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Ngn2 (Neurog2 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

88 Articles

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