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pAWP78-PVCpnf_Δpvc10

Plasmid

#198448

Purpose

Produces PVCs containing truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_Δpvc10

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPGK-empty

Plasmid

#35094

Depositor

David Segal

Article

Bhakta et al Methods Mol Biol. 2010;649:3-30.

Insert

deletion of zinc fingers and nuclease

Tags

HA

Expression

Mammalian

Mutation

use as a transfection balance for pPGK-ZFN plasmi…

Promoter

PGK

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-F98 IRES EGFP

Plasmid

#136618

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X FLAG

Plasmid

#225332

Purpose

Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ETV6_RUNX1

Plasmid

#205818

Purpose

Express mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_RUNX1 (ETV6, RUNX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

Plasmid

#157744

Purpose

knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-T7-6h-GST-α-Synuclein

Plasmid

#225224

Purpose

Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

GST
α-Synuclein

Tags

6xHis Tag and TEV Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-3xMBT

Plasmid

#46987

Purpose

Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Article

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

contains amino acids 190–530 of accession NP_0562…

Promoter

tac

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0751

Plasmid

#185628

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Flag-PGC1a-6His

Plasmid

#67637

Purpose

AAV vector expressing PGC1a gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

PGC1a (Ppargc1a Mouse)

Use

AAV

Tags

6XHis and Flag tag

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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TLS 4: hTLS-CHOP.pBSKS(+)

Plasmid

#21830

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS-CHOP (FUS Human)

Expression

Bacterial

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-Alpha-Actin-12

Plasmid

#54971

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Alpha-Actin (ACTA2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PML_RARA

Plasmid

#205897

Purpose

Express mEGFP-tagged fusion protein, PML_RARA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PML_RARA (PML, RARA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M

Plasmid

#140314

Purpose

Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-60 roGFP2-His

Plasmid

#65046

Purpose

Bacterial expression of His-tagged roGFP2

Depositor

Tobias Dick

Insert

roGFP2

Tags

His

Expression

Bacterial

Mutation

see sequence

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2528

Plasmid

#144127

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ERCC6 (ERCC6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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MEF2D-2A-neo

Plasmid

#190622

Purpose

Lentiviral expression plasmid of human MEF2D (long isoform), neomycin selection

Depositor

Junwei Shi

Article

Pingul et al iScience. 2022 Sep 15;25(10):105139. doi

Insert

MEF2D (MEF2D Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-pvc10

Plasmid

#243750

Purpose

For affinity purification of spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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