Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

327,804 results

Showing: 441 - 460 of 327804 results

  3. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

    Enlarge

  4. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

    Enlarge

  9. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

    Enlarge

  10. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

    Enlarge

  11. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid
    #210656
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] mouse IgG2a heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse IgG2a heavy chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210656-plasmid-map-sequence-id-416221

    Enlarge

  12. pTIPI_mK_Anti-HA [12CA5]

    Plasmid
    #210657
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] kappa light chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210657-plasmid-map-sequence-id-416222

    Enlarge

  13. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid
    #210656
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] mouse IgG2a heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse IgG2a heavy chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210656-plasmid-map-sequence-id-416221

    Enlarge

  14. pTIPI_mK_Anti-HA [12CA5]

    Plasmid
    #210657
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] kappa light chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210657-plasmid-map-sequence-id-416222

    Enlarge

  15. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (AAV Retrograde)

    Viral Prep
    #55639-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO hChR2(H134R)-EYFP (#55639). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO hChR2(H134R)-EYFP plasmid DNA. Ef1a-driven, Flp-dependent expression of ChR2(H134R)-EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  20. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

    Enlarge

Showing: 441 - 460 of 327804 results