322,390 results

1. eGFP-luciferase-C1

   Plasmid

   #163523

   Purpose

   Expresses both eGFP and firefly luciferase in mammalian cells

   Depositor

   Thomas Weber

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG BacMam N term StrepII eGFP 3C

   Plasmid

   #160683

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   StrepII eGFP 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEG BacMam N term StrepII eGFP 3C

   Plasmid

   #160683

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   StrepII eGFP 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Stephen Elledge, Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Thomas Westbrook, Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-TLR8-YFP

   Plasmid

   #13024

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 8 (TLR8 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-TLR8-YFP

   Plasmid

   #13024

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 8 (TLR8 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human SLC overexpression library

    Pooled Library

    #213694

    Purpose

    Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

11. Human SLC overexpression library

    Pooled Library

    #213694

    Purpose

    Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

12. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_ABEmax

    Plasmid

    #112095

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_ABEmax

    Plasmid

    #112095

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. evoBE4-SpRY

    Plasmid

    #226854

    Purpose

    Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. evoBE4-SpRY

    Plasmid

    #226854

    Purpose

    Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-SpRY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TLR2-CFP

    Plasmid

    #13015

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge