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179,553 results

Showing: 11501 - 11550 of 179553 results
  1. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Expression
    Plant
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pCMV-BE-zCas9

    Plasmid
    #101739
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV
    Available since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pSG5-HA-p300-DY1399

    Plasmid
    #89095
    Purpose
    mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p300-D1399Y mutant (EP300 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    p300-DY1399
    Promoter
    SV40
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA5-FRT-TO-EGFP-AID

    Plasmid
    #80075
    Purpose
    Mammalian expression vector for N-terminal EGFP-AID tagging
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-AID
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 11501 - 11550 of 179553 results