We narrowed to 9,924 results for: EPO;
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Plasmid#128230PurposeLamin A splicing reporterDepositorInsertlamin A (LMNA Human)
UseTagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huMcl-1.1
Plasmid#85530PurposeInducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporterDepositorInserthu Mcl-1.1 (MCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo
Plasmid#222636PurposeEzrin activation reporterDepositorInsertmiRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 XBB
Plasmid#212521PurposeEncodes SARS-CoV-2 variant XBB Spike for pseudovirus productionDepositorInsertSARS-CoV-2 Spike XBB (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.5
Plasmid#212523PurposeEncodes SARS-CoV-2 variant XBB.1.5 Spike for pseudovirus productionDepositorInsertSARS-CoV-2 Spike XBB.1.5 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-NR2F1
Plasmid#170698Purposedoxycycline-inducible overexpression of NR2F1DepositorInsertnuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationdeletion of 36G- please see depositor commentsPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1859A
Plasmid#169821PurposeExpresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation siteDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationS1859A; TCCC -> AGTC silent mutations at nt527…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GST-FBW7 alpha
Plasmid#197456PurposeThe plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.DepositorInsertFBW7 (FBXW7 Human)
UseIn vitro translationTagsGSTExpressionBacterialMutationSee depositor comments belowPromoterT7Available sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.25_S3-4-5-6
Plasmid#173956PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
Plasmid#78099PurposeNuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).DepositorInsertHistone H2B - Citrine (H2BC21 Human, Synthetic)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoter5xTetO pEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225590PurposeAAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB2-SV40-GFP (B2G)
Plasmid#65442PurposeExpression of mouse EPHB2 and GFP in mammalian cellsDepositorInsertEPHB2 (Ephb2 Mouse)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationSee depositor comments below.PromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1-IRES-NEO-Control
Plasmid#80757PurposeTransposon ReporterDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMutationPromoterEF1-IRES from PB-EF1-IRES-NEOAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin
Plasmid#99278PurposeDual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mVenus-IRES-mCherry
Puro
UseLentiviralTagsH2B, IRES, PGK, mCherry, and mVenusExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86
Plasmid#212538PurposeEncodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus productionDepositorInsertSARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]
Plasmid#122102PurposeAAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet
Plasmid#220608PurposeNeuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.DepositorInsert3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
UseAAV and Cre/LoxTags3x HA; ArgiNLSExpressionMammalianMutationPromoterhSynAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry
Plasmid#179437PurposeDual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertcoreHS4-TRE-cit-coreHS4-mch (DC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Promoter-hPE(+)-Luc
Plasmid#172003PurposeLuciferase Reporter for Human PTEN Enhancer PE - Forward OrientationDepositorInsertHuman PE sequence (hPE)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoterSV40 PromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Fgf4enhLV (FpG12)
Plasmid#69444Purposepositive control plasmid for lentiviral reporter expression in ESCsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_P278A
Plasmid#183113PurposeExpresses flag-tagged p53 with P278A mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine, see depositor com…PromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(RK)
Plasmid#27136DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationArginines in the SH2 domains mutated to lysines (…PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
p1.1-mCherry
Plasmid#162772PurposeFluorescent reporter for CHO expression studiesDepositorInsertred fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS-eGFP
Plasmid#162771PurposeFluorescent reporter for CHO expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterTagsExpressionMutationPromoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PKAsub-TagRFPT-CAAX
Plasmid#87712PurposeEncodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorInsertPKAsub-TagRFPT-CAAX
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2198
Plasmid#49347PurposeThis is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.DepositorInsertBmp2 promoter (shorter sequence) (BMP2 Human)
UseLuciferaseTagsluciferaseExpressionMutationPromoterBmp2 promoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR-miR-21-mut
Plasmid#53693PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationnucleotide sequence 1083-1086 is mutated to TTCGPromoterAvailable sinceJuly 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Tre-Tight ifgMosaic Donor vector (LH121)
Plasmid#99630PurposeTo clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxTagsExpressionMutation(Please see depositor comments below)PromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS1 INSIG2
Plasmid#60798PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sitesDepositorInsertINSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterthymidine kinase (HSV-TK promoter)Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL2-hOct4 DE-SV40-Luc
Plasmid#26279PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorInsertOct4 Distal Enhancer Element (DE) (POU5F1 Human)
UseLuciferaseTagsExpressionMammalianMutationSee Depositor Comments belowPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry
Plasmid#179429PurposeDual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-5kb-mch
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1
Plasmid#173947PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-20a-3p
Plasmid#103341PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-20a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-20a-3p target (MIR20A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIS1 mPELO
Plasmid#60805PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and mutated miR-155 sitesDepositorInsertPELO 3'UTR and mutated miR-155 binding site (PELO Human)
UseLuciferaseTagsExpressionMammalianMutationmutated miR-155 binding sitePromoterthymidine kinase (HSV-TK promoter)Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(FF)
Plasmid#27135DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationTyrosines in ITAMS 1-3 mutated to phenylalanines …PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS1 mATP2B1
Plasmid#60789PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and mutated miR-155 sitesDepositorInsertATP2B1 3'UTR and mutated miR-155 binding site (ATP2B1 Human)
UseLuciferaseTagsExpressionMammalianMutationmutated miR-155 binding sitePromoterthymidine kinase (HSV-TK promoter)Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-Y117C
Plasmid#110216PurposeExpression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationY117C, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only