167,948 results

1. pBTK527a

   Plasmid

   #110606

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pHR GFP-CaaX

   Plasmid

   #113020

   Purpose

   for lentivirus production of GFP-CaaX

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   GFP-CaaX
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPEE47

   Plasmid

   #139426

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mNeonGreen
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPEE46

   Plasmid

   #139425

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mRuby3
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWS481

   Plasmid

   #123029

   Purpose

   LexA UAS

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   LexO (6x) [2a]
   

   Expression

   Yeast

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGLuc

   Plasmid

   #135288

   Purpose

   GBPart - GLuc Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Gaussia Luciferase
   

   Use

   Luciferase and Synthetic Biology

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR CD19 tandem CAR

   Plasmid

   #113019

   Purpose

   for lentivirus production of CD19 tandem CAR

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Human, Synthetic, Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

   Viral Prep

   #171027-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Promoter

   EF1a

   Tags

   tdTomato

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

9. NLS-LgBiT-FRB

   Plasmid

   #183403

   Purpose

   Expresses nuclear-localized LgBiT-FRB

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-FRB
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-FLAG-S6K1

    Plasmid

    #100509

    Purpose

    Expresses FLAG tagged S6K1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIRT7 Flag

    Plasmid

    #13818

    Purpose

    Mammalian expression of human SIRT7 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT7 (SIRT7 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Missing exon 2

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pShuttle

    Plasmid

    #192871

    Purpose

    Gateway compatible vector with BsaI cloning site

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Available Since

    Dec. 12, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. repressilator (pZS1-lTlrLLtCL)

    Plasmid

    #26489

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    LacI, tetR
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST40-2XFL-Src-KD

    Plasmid

    #140313

    Purpose

    Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2/CMV-Luc/PGK-SB100X

    Plasmid

    #192869

    Purpose

    Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

    Depositor

    David Largaespada, Maria Castro

    Article

    Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

    Inserts

    luc
    SB100X
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

    Plasmid

    #69908

    Purpose

    Expresses a GFP circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_DLL1_WT

    Plasmid

    #82108

    Purpose

    Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DLL1 (DLL1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHIV-ODD-EGFP-IRES-dTomato

    Plasmid

    #87218

    Purpose

    lentiviral expression of ODD-EGFP

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a ODD domain (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ODD domain

    Promoter

    EF1

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. spNW80

    Plasmid

    #173487

    Purpose

    Expresses spNW80 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW80
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-CaMKIIa-mCherry

    Plasmid

    #114469

    Purpose

    mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Promoter

    CaMKIIa

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDAI-SceI-pheS

    Plasmid

    #111611

    Purpose

    Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Insert

    pheS
    

    Expression

    Bacterial

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

    Plasmid

    #70745

    Purpose

    for building 3-color reporter plasmid

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    mCherry-ssrA
    

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWKS11

    Plasmid

    #139418

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. attB-GFP

    Plasmid

    #65521

    Purpose

    Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. N-GFP-Dcp2

    Plasmid

    #163585

    Purpose

    express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    DCP2 promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUFlip-floxed2A-mRFP-; HE1A:BFP -2

    Plasmid

    #180009

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; HE1A: BFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOTTC1553 - pAAV EF1a EGFP-KASH

    Plasmid

    #131682

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    KASH

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. TetO-FUW-Lhx2

    Plasmid

    #61537

    Purpose

    Expresses Lhx2 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Lhx2 (Lhx2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. LcV2-Hygro

    Plasmid

    #91977

    Purpose

    LentiCRISPR v2 with Hygro cloned in place of Puro

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEntry_HCoV-OC43-nsp4

    Plasmid

    #168932

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-nsp4 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMKO.1 puro

    Plasmid

    #8452

    Purpose

    Retroviral vector for shRNA expression.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMA7CR_2.0

    Plasmid

    #73950

    Purpose

    pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Inserts

    cas9
    lambda beta
    dam
    recX
    

    Expression

    Bacterial

    Promoter

    pAra and pTet

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET30_Halotag_K73T_v2

    Plasmid

    #183692

    Purpose

    Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)
    

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDEST-CMV-C-EGFP

    Plasmid

    #122844

    Purpose

    C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti6.2-ccdB-Nanoluc

    Plasmid

    #87075

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CAG-mCherry-LacZshRNA

    Plasmid

    #73978

    Purpose

    LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the LacZ gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKM343

    Plasmid

    #69762

    Purpose

    Retroviral expression of CENP-I

    Depositor

    Iain Cheeseman

    Insert

    CENP-I (CENPI Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pAcGFP1-N1-CAHS3

    Plasmid

    #90031

    Purpose

    To express GFP-tagged CAHS3 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-HA-Nrxn1α-LNS2

    Plasmid

    #128106

    Purpose

    Express HA tagged Nrxn1α-LNS2

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS2 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge